油脂碱炼水洗废水酶法脱胶研究.PDF

16 2010, Vol. 31, No. 22 食品科学 ※工艺技术 1 2 2 1 2 , 魏贞伟 ，李红玲 ，王 玉 ，王俊国 ，于殿宇 * (1.吉林工商学院生物工程分院，吉林 长春 130062 ；2.东北农业大学食品学院，黑龙江 哈尔滨 150030) 对油脂碱炼过程中的水洗废水进行循环利用。将油脂碱炼废水进行离心分离，加入毛油中进行水化脱胶， 脱出水化磷脂后，进一步进行酶法脱胶，脱出非水化磷脂。通过单因素试验与正交试验，确定废水酶法脱胶的最 优工艺条件：水洗废水量 2.5% ，磷脂酶量30mg/kg ，温度55 ℃，pH4.9 ，时间3h ，搅拌速度90r/min 。在最优 条件下进行脱胶实验，测定脱胶油中的磷含量为 4.9mg/kg ，说明水洗废水可以替代软化水用于脱胶工序，每千克 成品油可节省热量 34～36kJ ，提高得率0.2% 以上。 毛油；水洗废水；磷脂酶；脱胶；节省热量 Application of Wastewater from the Alkaline Refining of Oil to the Enzymatic Degumming of Oil 1 2 2 1 2, WEI Zhen-wei ，LI Hong-ling ，WANG Yu ，WANG Jun-guo ，YU Dian-yu * (1. Branch of Biological Engineering, Jilin Business and Technology College, Changchun 130062, China； 2. School of Food Science and Technology, Northeast Agricultural University, Harbin 150030, China) Abstract ：In this study, the wastewater from the alkaline refining of oil was centrifugated, followed by the supernatant was then added to gross soybean oil for the removal of hydrated phosphatides, followed by further enzymatic degumming for the removal of non-hydrated phosphatides in order to achieve the recycle utilization of the wastewater. Based on single-factor and orthogonal array experiments, the optimal conditions for the enzymatic degumming with the wastewater were determined to be: wastewater loading, 2.5%; phospholipase A1 loading, 30 mg/kg; reaction temperature, 55 ℃; reaction pH, 4.9; and stirring speed, 90 r/min. The soybean oil degummed under the optimal conditions showed a phosphorus content of 4.9 mg/kg. This demonstrates that the wastewater from the alkaline refining of oil ca