内皮抑素-力达霉素辅基蛋白融合蛋白对乳腺癌胰腺癌和食管癌的靶.PDF

现代药物与临床 Drugs Clinic 第 30 卷 第 8 期 2015 年 8 月 ·917 · 内皮抑素-力达霉素辅基蛋白融合蛋白对乳腺癌、胰腺癌和食管癌的靶向性 研究 1 2 2 2* 姜文国 ，李 毅 ，张胜华 ，甄永苏 1. 滨州医学院 医药研究中心，山东 烟台 264003 2. 中国医学科学院 北京协和医学院 医药生物技术研究所，北京 100050 摘 要：目的 研究内皮抑素-力达霉素辅基蛋白融合蛋白（ES-LDP ）对人乳腺癌、胰腺癌和食管癌组织的靶向性。方法 分 别采用人体乳腺癌、胰腺癌和食管癌组织芯片，利用免疫组化的方法，研究 ES-LDP 与癌细胞的亲和力；并采用 Image-Pro Plus 6.0 软件计算吸光度值，进行统计分析。结果 与辅基蛋白比较，ES-LDP 对乳腺癌、胰腺癌和食管癌亲和力显著增高（P ＜ 0.001 ）。在胰腺癌组织，这种亲和力也显著高于对正常组织的亲和力（P ＜0.05 ）。结论 ES-LDP 对不同组织器官，亲和力 有所不同；在进行体内抗肿瘤活性评价时要注意选择合适的模型。 关键词：内皮抑素；力达霉素；内皮抑素-力达霉素辅基蛋白融合蛋白；组织芯片 中图分类号：R966 文献标志码：A 文章编号：1674 - 5515(2015)08 - 0917 - 04 DOI:10.7501/j.issn.1674-5515.2015.08.003 Targeting activity on breast cancer, pancreatic cancer, and esophageal cancer of endostatin-lidamycin fusion protein 1 2 2 2 JIANG Wen-guo , LI Yi , ZHANG Sheng-hua , ZHEN Yong-su 1. Medicine and Pharmacy Center, Binzhou Medical University, Yantai 264003, China 2. Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China Abstract: Objective To study the targeting activity on human breast cancer, pancreatic cancer, and esophageal cancer of endostatin- lidamycin fusion protein (ES-LDP). Methods Multiple tissue microarrays of breast cancer, pancreatic cancer, and esophageal cancer were used. ES-LDP and its affinity was studied by immunohistochemical way. Additionally, the cases were analyzed by Image-Pro Plus 6.0 software. The absorbance was log transformed and mainly performed statistical analysis. Results ES-LDP had higher affinity than LDP to breast cancer, pancreatic cancer, and esophageal cancer (P 0.001). Additionally, the affinity of ES-LDP to pancreatic tumor was higher than that of normal pancre