DNA芯片及其在医学上的应用.PDF

# 210 # Chinese Journal of Pathophysiology 2002, 18(2) : 210- 213 [ ] 1000- 4718(2002) 02- 0210- 04 DNA * 莫雪华, 周天鸿 ( , 510632) DNA chip and its applications in medicine MO Xue- hua, ZHOU Tian- hong (Dep artment of Biotechnology , College fo Lfi e Science and Technology , Jinan University , Guangzhou 516032, China A Review The evelopment of DNA chip has been strongly riven by mo ern approaches of life sci ence an microelectronics. The broa of DNA chip applications inclu e the etection of pathogens, the etec tion of mutations in genes involve in human iseases or affecte uring cancer progression, sequence analysis, the pattern of gene expression monitoring an pharmacy stu ies . This review explains the technology, its scope, an impact on me icine, as well as its cost an possible limitations. [ ] ; ; [ KEY WORDS] Gene expression; Biotechnology; Oligonucleoti e array sequence analysis [ ] Q75 [ ] A DNA DNA ( microarray) , , !!! , , DNA , , , , DNA , 92%- 94%, 30nt 1 DNA 20% ( post- synthesis- attachment ) DNA Southern blot , , , DNA , , , , ( robotic pin tool) , , , , 10 , DNA cDNA nL, 80- 100 m; ( pizoelectric ink- jet technology) , , , ( light - ir