Multiplex PCR analysis of Indica-japonica differentiation - 广西植物.PDFVIP

广 西 植 物 Guihaia Jul． 20 13 ，33 (4 ):460 －464 http :/ /journal． gxzw． gxib． cn DOI :10． 3969 /j ． issn． 1000-3142． 2013． 04． 006 ， ， ． PCＲ ［J］． ，2013 ，33 (4):460－464 刘志文 邹丹 陈温福 杂草稻叶绿体籼粳分化的多重 分析 广西植物 Liu ZW ，Zou D ，Chen WF． Multiplex PCＲ analysis of Indica-j ap onica differentiation of the chloroplast DNA in weedy rice ［J］． Guihaia ，2013 ，33 (4):460 －464 Multiplex PCＲ analysis of Indica-j ap onica differentiation of the chloroplast DNA in weedy rice 1，2 1 2* LIU Zhi-Wen ，ZOU Dan ，CHEN Wen-Fu (1． College of Bioengineering ，Dalian Polytechnic University ，Dalian 116034 ，China ; 2． Ｒice Institute ，Shenyang Agricultural University ，Shenyang 110161，China ) Abstract :The whole chloroplast genomes of the Indica cultivar 93-11 and Jap onica cultivar Peiai64S were analyzed and compared． A multiplex PCＲ marker of the chloroplast DNA OＲF100 and OＲF29-TrnCGCA region was optimized and con- structed． More than 200 weedy rice ，Asian cultivated rice and common wild rice accessions were then tested using the multiplex PCＲ． The results showed that there were obvious chloroplast DNA indica-j ap onica differentiations amongst the weedy ，Asian cultivated and common wild rice accessions． Furthermore ，the differentiations in weedy rice were correlated to the collected regions that were in accordance with the south indica type and north j ap onica type of cultivated rice in China． It was suggested that the j ap onica line of weedy rice should evolve from degraded cultivated rice or the j ap onica varieties (as the female parent)natural hybridization with other oryza materials for there was no wild form in north