高体革鯻消化道发生的组织学观察骆豫江12陈昆慈1朱新平1.DOC

高体革鯻消化道发生的组织学观察 骆豫江1,2，陈昆慈，朱新平1，潘德博1，常藕琴1，李凯彬1 （1．中国水产科学研究院珠江水产研究所,广东 广州 510380； 2．上海海洋大学水产与生命学院，上海 201306） 摘要：利用形态学和连续组织切片技术，对出膜后1-30d的高体革鯻消化系统胚后发育的组织形态学进行了系统观察和研究。研究表明：培养水温26.6～29.7 ℃出膜30h仔鱼张开，相通在显微镜下可见蠕动3d仔鱼Scortum barcoo Abstract：Histological changes of the digestive system and its associated glands were studies in Jade perch, Scortum barcoo from the first day (first day post-hatch dph) to 30 dph. Specimens for this study were hatched from artificially spawned broodstock and maintained in the indoor plastic tanks(26.6～29.7 ℃). The newly hatched larvae had one big yolk sac, and their digestive tract was only a columniform diverticulum, without communication with outside. Digestive tract communication with outside in 30-hour-old larva and their mouth shut up. 2-day-old larvae’s gut started crumple and peristalsis. The intestinal had been divided into anterior intestine and posterior intestine by intestinal valve. 3-day-old larvae could be fed, the epithelial of digestive tract begun differentiation, mucous cells appeared in esophageal, pancreas and liver appeared. The nutrition of fish began transferred to the stage of mixed nutrition. In this stage, the digestive tract was differentiated into five portions: buccal-pharyngeal cavity, esophagus, stomach, anterior intestine and posterior intestine, liver and pancreas had also been formed; these organs already had initial structure and the capacity of digestion and absorption. Along with the development of larvae fish, digestive system tended to perfect. Gastric glands appeared in the stomach of 21-day-old juvenile, marking the beginning of juvenile Key words: Scortum barcoo; digestive system; organogenesis 高体革鯻(Scortum barcoo)Jade perch），原产于澳大利亚格拉斯敦巴库河（Gladston’s Barcoo）上游至昆士兰东海岸一带的流域[1-4]鲈形目（Perciformes）、鯻科(Terapontidae)、革鯻属(Scortum)高体革鯻肉鲜美，营养丰富，无肌间刺，对饵料要求不高，易养殖生长速度快优淡水。于2001年4月首次从澳大利亚引进，经过几年的驯养和培育，于2004年进行人工繁殖试验获得成功在国内，，实验为高体革鯻，1.5～2kg。5月下旬采用人工的方法获得受精卵。,s固定液固定24小时，然后用50%的酒精保存。1～29天的仔稚鱼进行了石蜡包埋、切片，1～13d的样品切片厚度3μm，