黄淮海地区主要玉米自交系的SSR遗传多样性分析.pdfVIP

玉 米 科 学2008，16(2)：1～7 Journal of Maize Sciences 文章编号：1005-0906(2008)02-0001-07 黄淮海地区主要玉米自交系的 SSR遗传多样性分析 肖木辑1， ，李明顺 ，李新海 ，张世煌 (1．中国农科院作物科学研究所玉米中心，北京 100081；2．沈阳农业大学，沈阳 110161) 摘 要：利用7O对SSR引物研究了我国黄淮海地区63份玉米自交系的遗传多样性。共检测出277个等位基 因变异，每对引物检测等位基因2～7个，平均3．96个，每个位点的多态性信息量介于O．177～O．827，平均O．581。63 份自交系之间的遗传相似系数变化范围在O．62～O．9l。聚类分析表明，63份自交系被划分为4个群。在黄淮海地区 利用的骨干种质为PA(Reid)、塘四平头(D)和PB(non—Reid)，杂种优势模式主要为PA×塘四平头。群内平均遗传相似 系数高于群间的平均遗传相似系数，生产上主要推广杂交种的亲本自交系大多来自不同杂种优势群。提供27对区 分能力强的SSR引物，用于供试自交系的快速聚类，结果与系谱来源及7O对引物的聚类结果基本一致。 关键词：SSR标记；玉米；自交系；遗传多样性 中图分类号：$513．024 文献标识码：A Genetic Diversity Revealed by SS R Markers Among I nbred Lines Used in Summer Maize Breeding PrOgram in Huanghuaihai Area of China XIAO Mu-ji ，LI Ming—shun ，LI Xin-hai ，ZHANG Shi-huang 1．Institute ofCrop Science，AMBIoNET—Ch Lab，Chinese Academy ofAgricuhural Sciences，Be~iing 100081； 2．ShenyangAgricuhural University,Shenyang 1 10161，China) Abstract：Seventy SSR primers were used to detect genetic diversity among 63 inbred lines，which were widely used in summer maize breeding programs in Huanghuaihai area of China．Two hundreds and seventy—seven polymor- phic fragments were detected．The average number of allele per SSR locus was 3．96 with a range from 2 to 7．The polymorphism information content(PIC1 for SSR loci varied from 0．177 to 0．827 with an average of 0．581．Genetic similarity coefficient am ong the 63 inbred lines ranged from 0．62 to 0．91．These inbred lines could be classified into 4 distinct clusters．The dominant germplasm were PA(Reid)，Sipingtou(D)and PB(non-Reid)clusters．The dominant heterotic pattern was PA X Sipingtou．Genetic similarity coefficients am ong clusters were higher than that within clus— ters．Mo