大孔树脂对槐角总黄酮分离纯化工艺研究.pdfVIP

四 川 中 医 2008年第26卷第2期 · 40 · Joamal of Sichuan of Ih出tional Chinese 1Mecficine Vo1．26，No．2，2008 大孔树脂对槐角总黄酮分离纯化工艺研究 袁崇均 王 笳△ 陈 9『9 罗 森 吴 燕 四川省中医药科学院 (列川成郜610041) 摘要：目的：研究大孔吸附树脂分离纯化槐角中总黄酮的工艺条件及参数，使分离工艺达到最优化。方法：以槐 角总黄酮的吸附量和解吸率为指标筛选大孔吸附树脂，用紫外分光光度法测定槐角总黄酮含量对所筛选树脂分离纯化 槐角总黄酮工艺进行评价。结果：Dll】1型大孔树脂对槐角总黄酮的吸附性能和解吸性能较好，静态饱和吸附量为 67．78rag·g一，解吸率为97．58％；其吸附纯化奈件为槐角提取物上样浓度为5．Omg·g一，吸附流速为2．OBV·h f树脂 床体积)，以7oo／o乙醇作为洗脱荆进行洗脱效果最佳。经D】{J1树脂纯化后槐角总黄酮的含量为67．3％。结论：该法简 单可行，分离效果好，能满足于大生产的要求。 关键词：槐角 总黄酮 大孔吸附树脂 分离纯化 中图分类号：R284．2 文献标识码：A 文章编号：1000—3649(2008)02一O04O一03 Study on Separation and Purification ofTotal Flavones from Huaijiao by Macroporous Adsorption Resin／Yuan Cho,~jun／／ SichuanAcademy ofChineseMedicine Science(Chengdu Sichuan 610041。China) Abstract objective：To optimize the purification method of the total flavones in Huaijiao by lIhacroporous resin．Methods：The static adsorption／desorption characteristics were taken as index to select the macmpomus resin．rI11e separating efficiency WaS evaluated by meaSuring the concen~ation of total flavones in Huaijiao with IⅣspeetrophotometer．Results：The D101 macroparous resin had the best separating efficiency．The static adsorption capacity／desorption ratio ofthe total flavones were 67．78mg’g一／97．585％ ．When the concentration of the HuaJjiao extract sample reached 5．0mg／mL。the current velocity ofadsorption 2．OBV． 1 f resin bed vol— ume)，and 70％ ethanol as eluant，the purity of total flavones could reach 60％．Conchlsion：The method is simple and feaSible。 with good separating effect which call meet industrial requirements． Key words Huaijiao，total flavones，mavroporous resin，separation and purification 槐角为豆科植物槐 Sophora Japorica L．的干燥成 (购于成都市五块石中药材市场，经本院资源研究所 熟果实，其作为常用中药，始载于《神农本草经》，