involvement in iron–sulphur cluster assembly - citeseerx.pdfVIP

Comparative and Functional Genomics Comp Funct Genom 2004; 5: 328–341. Published online in Wiley InterScience (). DOI: 10.1002/cfg.406 Research Paper Evolution and cellular function of monothiol glutaredoxins: involvement in iron–sulphur cluster assembly 1 1 ´ ´ 1 ´1 2 Felipe Vilella , Rui Alves , Marıa Teresa Rodrıguez-Manzaneque , Gemma Bellı , Swarna Swaminathan , Per Sunnerhagen2 1 and Enrique Herrero * 1 ` ` ` Departament de Ciencies Mediques Basiques, Facultat de Medicina, Universitat de Lleida, Rovira Roure 44, 25198-Lleida, Spain 2 ¨ ¨ Department of Cell and Molecular Biology, Goteborg University, PO Box 462, S-40530 Goteborg, Sweden *Correspondence to: Abstract Enrique Herrero, Dept. de Ciencies Mediques Basiques, A number of bacterial species, mostly proteobacteria, possess monothiol glutaredoxins Facultat de Medicina, Universitat homologous to the Saccharomyces cerevisiae mitochondrial protein Grx5, which is de Lleida, Rovira Roure 44, involved in iron–sulphur cluster synthesis. Phylogenetic profiling is used to predict 25198- Lleida, Spain. that bacterial monothiol glutaredoxins also participate in the iron–sulphur cluster E-mail: enric.herrero@cmb.udl.es (ISC) assembly machinery, because their phylogenetic profiles are similar to the profiles of the bacterial homologues of yeast ISC proteins. High evolutionary co- occurrence is observed between the Grx5 homologues and the homolo