幸国药理学报t h r c.pdf

BIBLID ISS ActaPharmacologicaSin[ca 幸国药理学报 M orphineinhibited respiratoryburstofneutrophils andscavenged oxygen freeradicals CHEN Shu—Yuan，HUO Qi—Lu，HUANG JianBang，CHEN zhi—Liang．SHIXing—Hua，LIAO Xi—Ling (DepartmentofPharmacology．NanfangHospital，FirsfMilitaryMedicalUniversity， Guangzhou 510515，China) AIM !Tostudytheeffectsofmorphineonac raised the possibility that the immunosup— tireoxygenfreeradicals． M ETHOD ：Chemi一 pressanteffectofmorphinewasrelated toits 1uminescencemethod wasusedtomeasure(a) freeradicalsscavengingaction． Thepresent activeoxygengenerationinducedbyrespirato— studywasundertaken to examineby chemilu— ry burstofneutrophils from human blood minescencemethodtheeffectsofmorphineon stimulated with phorbo1 myristate acetate (a)therespiratoryburstofneutrophilsstimu— (PMA)，(b)Superoxideanion (O；)induced Iatedwithphorbolmyristateacetate (PMA)， byxanthine—xanthineoxidasesystem ，(c)h (b)superoxidegeneratedhyxanthineoxidase droxylradical (·OH)generated by ascorbic system ， (c) hydroxylradicalproduced by acid (AA)一Cu”一zymosan，and (d)therelease ascorbic acid (AA )一Cu zymosan system ， ofH，O，． RESUL1S：The (a)，(b)，(c)，and and (d)releaseofhydrogenperoxide． (d)werescavengedbymorphineandtheirme dian inhibitoryconcentrations (IC50and 95 MATERIALS AND M ETHoDS confidencelimits)were21．1 (13．0～ 34．0)， Drugs Morphinehydrochloride (M ar，Shanghai 54．1 (5O．0— 58．5)，224．0 (128．2— 390．8)， Pharmceuticallot № 890212)． fresh adult blood and 66．9 (62．9— 71．0)，nmolL～ ，respec— (GuangzhouBloodBank)；Luminol，PM Atxanthine， tively， C0NCLUS10N ： The immuno— xanthineoxidase，zyTi3osan，HEP