1型嗜肺军团菌和其他常见肺炎致病菌共同抗原的研究.pdf

674 2007 2 7 4 Chin J Misdiagn Feb, 2007 Vol 7 No. 4 【研究原著】 1 张兴虎, 齐玉琴, 黄方, 万文辉 : 1 ( LP1) : LP1 , ; LP 1 ; ( - ) ; ( - ) : - : SDS PAGE Western blot Western blot 154 kDa 97 kDa 79 kDa 5 kDa 44 kDa LP 1LP1 : LP 1 3 , 2 , 1, , / ; , / ; , / ; , / R446. 51A 1009-6647(2007) 04-0674-03 Research of Co-antigen of Legionella Pneumonia Sergroup 1 and Other Frequent Pathogenic Bacteria of Pneumonia ZHANG Xing-hu, QI Yu-qin, HU ANG Fang, et al. (D ep art ment of Ger iatr ics, Na nj ing Gener na l H osp it al of N anj ing Command ,N anj ing 21002) AbstractObjective: To study whether there w ere the same antigens betw een Legionella pneumonia serogroup 1 and the other pathogenic bacteria of pneumonia. Methods:Tw o rabbits w ere immunized w ith the somat ic antigens of Legionella pneum ophila serogroup 1( LP 1) , the serum w ere separated from the w hole blood used as the anti-LP1 antibody.T he bacteri- 1 ( 1) , . al somatic antigens were ext racted including Legionella pneumophila sergroup LP and Bacillus coli and so on All these antigens w ere separated by SDSgel electrophoresis, then test ed by Western-blot assay . Results:The Western-blot as- say showed that there were the same bands in the lanes of Staphylococcus epidermidis, hemophilies influenzae and Pseu- 154 97 , domonas pyocyanea at the position of kDa and kDa in the lanes of hemophilies influenzae and Pseudomonas py- 79 , , , ocyanea at the position