camkⅱ表达调控对大鼠肝移植术后肝功能的影响.pdfVIP

• 438 • Chinese Journal of Bases and Clinics in General Surgery, Apr. 2017, Vol. 24, No.4   ·论 著· CaMK Ⅱ表达调控对大鼠肝移植术后 肝功能的影响 冉江华，郑克谱，李望，张熙冰，刘驳强，李温凯 昆明医科大学附属甘美医院暨昆明市第一人民医院肝胆胰血管外科（昆明  650011 ） 【摘要】   目的    探讨钙调素依赖蛋白激酶家族（calmodulin-dependent kinases casades ，CaMK ）Ⅱ表达调控对 大鼠肝移植术后肝功能的影响。 方法    采用经典二袖套法建立 SD 至 SD 大鼠同种异体原位肝移植模型，并构建 CaMK Ⅱγ 蛋白和 CaMK Ⅱγ shRNA 的慢病毒表达体系。分别将表达 CaMK Ⅱγ shRNA 的慢病毒载体及表达 CaMK Ⅱγ 蛋白的慢病毒载体灌注至肝移植术后大鼠体内，同时予以灌注相应的空载体及生理盐水形成对照，分 别于术后不同时间点取大鼠下腔静脉血测定其血清 ALT 及 AST 水平。 结果    灌注表达 CaMK Ⅱγ shRNA 的慢病 毒载体的移植术后大鼠血清 ALT 及 AST 降低（P0.05）；灌注表达 CaMK Ⅱγ 蛋白的慢病毒载体的移植术后大鼠 血清 ALT 及 AST 升高（P 0.05 ）；灌注空白载体及生理盐水的移植术后大鼠血清 ALT 及 AST 均无明显差异 （P0.05）。 结论    特异性阻断 CaMK Ⅱ信号通路可有效降低大鼠肝移植术后的血清 ALT 及 AST 水平；而增强的 CaMK Ⅱ信号通路则升高大鼠肝移植术后的血清 ALT 和 AST 水平。 【关键词】  钙调素依赖蛋白激酶家族；肝移植；肝功能；大鼠 Effect of CaMK Ⅱ expression regulation on liver function after liver transplantation in rats RAN Jianghua, ZHENG Kepu, LI Wang, ZHANG Xibing, LIU Boqiang, LI Wenkai Department of Hepatobiliary and Pancreatic Vascular Surgery, Affiliated Calmette Hospital of Kunming Medical University (The First Peoples Hospital of Kunming), Kunming, Yunnan 650011, P.R.China Corresponding author: RAN Jianghua, Email: rjh2u@163.com 【Abstract 】 Objective    To investigate the effect on expression regulation of calmodulin-dependent kinases casades (CaMK) Ⅱ on liver function after liver transplantation in rats. Methods    Allogeneic orthotopic liver transplantation model was established by using the classic two-cuff method. The lentiviral expression systems of CaMK Ⅱγ protein and CaMK Ⅱγ shRNA were constructed. The lentiviral vector expressing CaMK Ⅱγ shRNA and the lentiviral vector expressing CaMK Ⅱγ protein were perfused into the rat after liver transplantation respectively, and the corresponding blank vector and normal saline were perfused into the control group at the same time. The serum levels of