高效液相色谱法测定水果中的赤霉素、苯甲酸、糖精钠和胭脂红.pdfVIP

1330 中国卫生检验杂志2008年7月第18卷第7期 Chinese Journal ofHeaI血Laboratory Technology，Jul 2008；Vol 18 No 7 【化学测定方法】 高效液相色谱法测定水果中的赤霉素、苯甲酸、糖精钠和胭脂红 湛社霞，孙世宏，臧李纳，朱琳，吕杰 (广州市天河区病病预防控制中心，广州 510635) [摘要] 目的：建立高效液相色谱法测定水果中赤霉素、苯甲酸、糖精钠和胭脂红含量的方法。方法：采用溶剂萃取对样 品进行提取，以CH OH／H 0(NH Ac—HAc)为流动相，优化流动相配比、流速，采用二极管阵列检测器进行检测( = 210 nm)，利用反相高效液相色谱分析法测定水果中赤霉素、苯甲酸、糖精钠和胭脂红。结果：该法线性良好，相关系数r 0．999，相对标准偏差5．0％，加标回收率80％～95％。结论：本方法简单可靠、快速，灵敏度高，定量准确，可用于同 时测定水果中赤霉素、苯甲酸、糖精钠和胭脂红。 [关键词] HPLC；水果；赤霉素；苯甲酸；糖精钠；胭脂红 [中图分类号] 0657．7 2 [文献标识码] A [文章编号] 1004—8685(2008)07—1330—03 Determination of gibberellin，benzioc acid，saccharin sodium and ponceau 4R in fruits by high performance liquid chromatography Zhan She—xia，Sun Shi—hong，Zang Li—n0，Zhu Lin，Lv fie (Tianhe Center for Disease Control and Prevention，Guangzhou 510635，China) [Abstract] Objective：A method for the determination of gibberellin，benzoic acid，saccharin sodium and ponceau 4R in the fruits by HPLC was established in this research．Methods：Samples were extracted by solvent extraction，and mobile phase was CH3 OH／H2 O(NH4 Ac—HAc)=35：65．Flow rate is 0．6 m]／min and samples were determined by DAD detector．The column temperature was controlled at 30℃．Results：The method was able to separate and determinate 4 materials in fruits easily and ef- fectively．In the experiment，good relative(r0．999)，broad linearity range，low detection limit，good analytic accuracy and pre— cision were obtained(RSD5％ ．The average recoveries were between 80％and 95％．Conclusion：This method is simple，rap— id，sensitive and accurate．It is suitable for simultaneous determination gibberellin，benzioc acid，saccharin sodium and ponceau 4R in fruits． [Key words] HPLC；Fruits；Gibberellin；Benzioc acid；Saccharin sodium；Ponceau 4R 赤霉素是一种广谱性植物生长调节剂，几乎在植物生长、 法主要有高效液相色谱法 、薄层色谱法和离子选择电极法； 发育的各个阶段都起着调节作用，尤其对水果等有着显著的