新生儿脐血细胞因子及特应质家族相关性探究.docVIP

新生儿脐血细胞因子及特应质家族相关性探究【摘要】目的:测定并分析新生儿脐血IL-4，IL-13，IFN-γ，TGF-β的含量与特应性家族的相关性。方法:应用ELISA法检测32名父母均非特应质婴儿、24名父母单方特应质婴儿及16名父母双方均为特应质婴儿脐血IL-4，IL-13，IFN-γ，TGF-β含量，并随访6个月内婴儿发生湿疹、食物过敏性腹泻及喘息等特应质疾病的情况。结果:单方特应质组和双方特应质组的IL-4，IL-13均明显高于非特应质组（P＜0.01,P＜0.05）；双方特应质组的IL-4，IL-13均明显高于单方特应质组（P＜0.05）。TGF-β含量单、双方特应质组明显低于非特应质组（ t＝2.1471，2.4717， P＜0.05）；随访婴儿30人发生湿疹,总发病率41.67%,是婴儿期最常见的特应性疾病；随访婴儿中7人发生食物过敏性腹泻,总发病率9.72%；随访婴儿中发生喘息人数为8人,总发病率11.11%。非特应质组发生特应性疾病总人数8人（25%）,湿疹8人,食物过敏性腹泻1人,喘息1人； 单方特应质组发生特应性疾病总人数13人（54.17%），湿疹10人,食物过敏性腹泻3人,喘息3人；双方特应质组发生特应性疾病总人数14人（87.5%），湿疹12人,食物过敏性腹泻3人,喘息4人。单方特应质组和双方特应质组的婴儿发生特应性疾病的人数均高于非特应质组（P＜0.05）；双方特应质组的婴儿发生特应性疾病的人数高于单方特应质组（P＜0.05）。结论:特应性疾病有遗传倾向，脐血细胞因子检测对预测特应性疾病发生有一定临床意义。 【关键词】脐血；细胞因子；特应质 【中图分类号】R722.1【文献标识码】A【文章编号】1008-6455（2011）04-0009-02 【Abstract】Objective:To survey and analyze the content and the possible risk factors of IL-4，IL-13，IFN-γ，TGF-β in cord blood of new arrivals. Methods:The cord serum of 72 full-month, normal born, healthy new arrivals in qingdao were analyzed using ELISA for the content of IL-4，IL-13，IFN-γ，TGF-β.The allergic diseases of parents and the occurence of atopic diseases of infants within 6 months were also recorded. Results:The content of IL-4 and IL-13 in cord serum in the group of allergic families was significantly higher than the control group （P＜0.01,P＜0.05）,while the content of TGF-β in cord serum in the group of allergic families was significantly lower than the control group （P＜0.01,P＜0.05）. The overall incidence rate of eczemawas 41.67% in 30 follow-up infant；Food allergy overall incidence of diarrhea was 9.72%in 7 follow-up infant；Overall incidence of wheezing was 11.11%the incidence of Atopic diseases, was 25% in the control group,8 cases of eczema, 1 cases of food allergy diarrhea, 1 cases of wheezing; the incidence of Atopic diseases, was 54.17%in One parent quality of infant atopic group,10 cases of eczema, 3 cases of food allergy diarrhea, 3 cases of wheezing ;the incidence of Atopic diseases, was 87.5%in Both pare