草酸脱羧酶促进草酸钙结晶溶解的体外研究.pdfVIP

※生物工程 食品科学 2015, Vol.36, No.19 159 草酸脱羧酶促进草酸钙结晶溶解的体外研究 1 1 2, * 1 1 1 2 贺俊斌 ，龙 寒 ，林日辉 ，梁宇薇 ，陈阳阳 ，高 华 ，黄奇良 （1.广西民族大学海洋与生物技术学院，广西高校微生物与植物资源利用重点实验室，广西 南宁 530006 ； 2.广西民族大学化学化工学院，化学与生物转化过程新技术广西高校重点实验室，广西 南宁 530006 ） 摘  要：在体外实验条件下，考察了加酶量、反应pH值、反应温度及其产物甲酸根等因素对草酸脱羧酶催化草 酸降解从而促进草酸钙结晶重新溶解过程的影响。结果表明，在10 mL反应体系中，添加4 U草酸脱羧酶，反应 pH 3.0 37 1 168 h 5% ，反应温度为 ℃，反应进行 周（ ），可使近 的草酸钙结晶重新溶解；实验结果表明，草酸降解 产物甲酸根对草酸脱羧酶促进草酸钙结晶溶解产生抑制作用。 关键词：草酸脱羧酶；草酸钙；溶解 Oxalate Decarboxylase Promotes the in vitro Dissolution of Calcium Oxalate Crystals 1 1 2, * 1 1 1 2 HE Junbin , LONG Han , LIN Rihui , LIANG Yuwei , CHEN Yangyang , GAO Hua , HUANG Qiliang (1. Guangxi Colleges and Universities Key Laboratory of Utilization of Microbial and Botanical Resources, School of Marine Sciences and Biotechnology, Guangxi University for Nationalities, Nanning 530006, China; 2. Key Laboratory of New Techniques for Chemical and Biological Conversion Process, School of Chemistry and Chemical Engineering, Guangxi University for Nationalities, Nanning 530006, China) Abstract: Crystallization of calcium oxalate in the urinary system is one of the important causes of urinary stones, and enzymatic degradation of oxalic acid for preventing calcium oxalate stones has become a research hotspot. Under in vitro experimental conditions, the factors that affect the re-dissolution of calcium oxalate crystals catalyzed by oxalate decarboxylase were studied. The results indicated that when 4 U of