再接种内生真菌对葡萄叶内生真菌群落结构的影响.PDFVIP

微生物学通报 Dec. 20, 2014, 41(12): 2458−2465 Microbiology China © 2014 by Institute of Microbiology, CAS tongbao@ DOI: 10.13344/j.microbiol.china.140209 研究报告 再接种内生真菌对葡萄叶内生真菌群落结构的影响 Δ Δ * 马勉娣 张秀英 程玉 黄治钰 张汉波 杨明挚 ( 650091) 摘 要【目的】探讨回接内生真菌对葡萄叶内生真菌群落结构的影响。【方法】将葡萄叶中分 离到的8 株内生真菌回接到健康生长的玫瑰蜜葡萄叶片上，同时设置管理上喷施农药与未喷施 农药两组处理，3 个月后分析不同真菌菌剂处理后葡萄叶片内生真菌的分离率、优势度和多样 性指数。【结果】5 种菌剂的处理(Xylaria sp.、Nigrospora sp.、Alternaria sp. 1、Alternaria sp. 2 和Colletotrichum sp.)在处理后的叶片中分离到了较多数量的接种菌。不同菌剂处理后葡萄叶片 菌群结构差异显著。【结论】与对照处理的葡萄相比，菌剂的处理增加了叶内生真菌的分离率， 但显著降低了内生真菌的多样性指数；发现喷施农药组叶片内生真菌的分离率较低，但多样性 相对较高。 关键词葡萄，再接种，内生真菌，菌群结构 Effects on folia endogenous flora structure of grape after re-inoculation with different endophytic fungi MA Mian-DiΔ ZHANG Xiu-YingΔ CHENG Yu HUANG Zhi-Yu * ZHANG Han-Bo YANG Ming-Zhi (School of Life Sciences , Yunnan University, Kunming, Yunnan 650091, China) Abstract: [Objective] The research is to study the effects of endophytic fungi re-inoculation on folia endogenous flora structure of grape. [Methods] Eight strains of endophytic fungi which isolated from wine grape leaves were re-inoculated to the healthy leaves of grapevine (variety: Rose-honey), and the effects on the folia endophytic fungi structure of grape were analyzed. Meanwhile, effects differences on the endophytic fungi structure to both groups of grapevine with and without pesticide after endophytic fungi re-inoculation, were also studi