黄檗中生物碱含量的高效液相色谱分析 - 林产化学与工业.pdf

24 V ol. 24 Supplement 2004 8 Chem istry and Industry of For est Pr oducts A ug. 2004 1 2 1 1* 秦彦杰 , 张玉红, 王 洋 , 阎秀峰 ( 1. 东北林业大学生命科学学院, 黑龙江 哈尔滨 150040; 2. 东北林业大学森林植物生态学教育部重点实验室, 黑龙江 哈尔滨 150040) Q IN Y J : ( Phellodendron am urense Rupr . ) : K YA HIQ sil C 18 ( 250 mm 4 . 6 mm , 5 m) , ( 1 000 mL 3. 4 g 1. 7 g ) 11, 1. 0 mL min, 34 5 nm , 10 L 63 % , 41 64 minHPLC , : , , , , , : ; ; ; : T Q 283; S792 . 3 1 : A : 0253- 2417( 2004) S0- 0 115- 04 DET ERM INA T ION OF AL KA LO IDS CONT EN T IN PH EL L ODEN DR ON A M UR EN SE RU PR. BY H PL C 1 2 1 1 QIN Y an-jie , ZHAN G Y u- hong , WAN G Y ang , Y AN X iu-f eng ( 1. College of L fi e Sciences , N or heas Fores ry Univ ers i y , H ar bin 150040, Ch ina; 2. K ey L abora ory of Plan Fores ry Ecology , N or heas Fores ry Univ ers i y , H ar bin 150040, Ch ina) Abstract: A simple, rapid and sensitiv e metho d for the determination of alkaloids content in Phellodendron am ur ense R. w as established . Alkaloids w as ult rasonically extracted w ith 63 % alcohol at 41 for 64 min , and determined by high performance liquid chromatog raphy. T he alkaloids w ere separated on a KYA HIQ sil C 18 column ( 250 mm 4. 6 mm , 5 m) , using acetonitrile- aqueous solution ( 3 . 4 g K H PO and 1. 7 g SDS i