邻苯二甲酸二(22乙基己基)酯酶促降解性的研究-环境科学学报.PDF

21 1 Vol.21, No. 1 2001 1 A TA S IENTIAE IR UMSTANTIAE Jan.,2001 :025322468(2001)20120013205 :X172 :A (22) 1 2 3 3 4 1 , , , , , ( 1. , 510275;2. ;3. ;4. ) :FS1(P seudomona s f luor escens FS1), P . f luo2 r escens FS1(22 )(DEHP) . P . f luor escens FS1 DEHP , P . f luor escens FS1.P . f luor escens FS1DEHP pH 6. 5) 8. 0, 25) 35e , K m 190.48nmol/ mL. , DEHP P . f lu or escens FS1, , , O H O. 2 2 :(2- );; ; Study of enzymatic degradability of di(22ethylhexyl)phthalate 1 2 3 3 4 1 ZENG Feng , KANG Yuehui , FU Jiamo , SHENG Guoying , YANG Huifang ,Zhang Zhanxia ( 1. School of hemistry and hemical Engineering, Zhongshan University, Guangdong 510275; 2. Research enter for Eco2 Enviro2 mental Science, hinese Academy of Sciences; 3. State Key Laboratory of Organic Geochemistry, Guangdong Key Laboratory of Environment Protection and Resources Utilization, Guangzhou InstituteofGeochemistry, hineseAcademy Sciences; 4. Instituteof Microbiology, hineseAcademy of Sciences) Abstract:Degradative characteristics ofdi(22 ethylhexyl)phthalatewere determinedby thecrudeenzyme extracted from P seudomonas f luor escens FS1 which was isolateed from the activatedsludgeat a petrochemical factory. The results showed that the PAEs2degrad2 ing enzyme, mainly locatizedin solublepart ofcytoplasm, is an intracllular enzyme. Theoptium temperatureandpH ofthePAEs2 de2 grading enzyme activity to di(22 ethylhexyl)phthalate are 20) 35e and 6. 5) 8. 0, respectively .The Michaelis2 mentn. s constant (K m) of di(22ethylhexyl)phthalate enzymatic degradation