克拉维酸高产菌的选育-河北工业科技.PDF

28 3 河 北 工 业 科 技 Vol. 28, No . 3 2011 5 H ebei Journal of Industrial Science and T echnolog Ma 20 11 : 1008- 1534( 2011) 03-0149- 04 ( 石药集团河北中润制药有限公司, 河北石家庄 050041 ) : 以带小棒链霉菌bs1574 为出发菌株, 经 线照 , 并结合甘油耐受性菌株的理性筛选, 选育得到较佳诱变菌株bs2325, 效价达2 569 g/ mL采用摇瓶发酵初筛和复筛, 选育出甘油耐受 性正向突变菌株, 是出发菌株( 效价为968 g/ mL) 的2. 65 倍, 该突变菌株在琼脂斜面培养基上连 续转接传代 4 代, 克拉维酸的产量保持稳定 : 克拉维酸; 线诱变; 耐甘油; 选育 : R738 : A Breeding of clavulanic acid mutant ZHANG Ru-ling ( H ebei Zhongrun Pharmaceutical Compan Limited, CSPC, Shijiazhuang H ebei 05004 1, China) Abstract: The met hod of screening and selecting bett er mutants of bs2325 b -ra irradiat ion onto st reptom ces bs1574 is put forw ard, and the titer is 2 569 g/ mL . B fermentation screening and secondar screening, st rains of mutant gl cerol positive tolerance is selected, w hose titer is 2. 65 times that of the original strain( 968 g/ mL) . T he mutant st rain grows w ell during four generations on the agar slant medium, and the ield of clavulanic acid remains stable. Key words: clavulanic acid; -radial mut agenesis; gl cero-l resistant ; breeding ( clavulanic acid, CA ) - -( 5, 6, 7) , , ( streptom ces [ 1] clavuligerus) , , , , , [ 3] - , , - , [ 2] , , , , 834. 8 g/ mL[ 4] , , bs 1574 , , 3 , , , , bs2325 , 2. 65 , : 2011-01- 10; : 2011- 03- 11 : 1 : ( 2007BAI26B07) : ( 1970- ) , ,, , 1. 1 bs 1574,