异丙酚麻醉损害恐惧记忆的巩固但不影响恐惧记忆的再巩固尹骏陆菡.DOC

异丙酚麻醉损害恐惧记忆的巩固但不影响恐惧记忆的再巩固 基金项目： 作者单位：200025 上海，上海交通大学医学院附属瑞金医院麻醉科（尹骏、陆菡、薛庆生、张富军、于布为）；华东师范大学脑功能基因组学教育部重点实验室（马婧、曹晓华） 通讯作者：张富军，电子邮箱为 fujunzhang1964@163.com 【摘要】实验包括两部分：异丙酚麻醉对恐惧记忆巩固的影响和异丙酚麻醉对恐惧记忆再巩固的影响。其中，异丙酚麻醉对恐惧记忆巩固的影响又可分为异丙酚麻醉剂量对恐惧记忆的影响及异丙酚麻醉时刻对恐惧记忆巩固的影响。在研究异丙酚麻醉剂量对恐惧记忆巩固的影响时，我们将ml/kg，异丙酚100mg/kg，异丙酚150mg/kg，异丙酚200mg/kg；24小时后进行恐惧记忆测试且在测试之前先进行开场实验。在研究异丙酚麻醉时刻对恐惧记忆巩固的影响时，我们将40只ml/kg的生理盐水；h，1h，2h，3h接受腹腔注射150mg/kg异丙酚麻醉；24小时进行恐惧记忆测试，同样在测试之前先进行开场实验。在研究异丙酚麻醉对恐惧记忆再巩固的影响时，我们将24只成年雄性r组和r组；r组和r组大鼠分别在场景恐惧再现完成后即刻接受腹腔注射15ml/kg生理盐水或腹腔注射150mg/kg异丙酚；24小时后进行场景恐惧记忆测试，依然在测试之前先进行开场实验。 结果 各组实验大鼠在开场实验600s中的运动速度，运动距离，中央区时间均无统计学差异（）；在场景恐惧训练结束后即刻接受较高剂量（150mg/kg或200mg/kg）的异丙酚麻醉会损害场景恐惧记忆的巩固（P值均0.05）；在场景恐惧训练结束后1h，2h，3h接受150mg/kg的异丙酚麻醉均不会损害场景恐惧记忆的巩固。场景恐惧记忆再现完成后即刻接受150mg/kg异丙酚麻醉不会影响恐惧记忆的再巩固过程。 【关键词】异丙酚；记忆巩固；记忆再巩固 Propofol could impair the consolidation of fear memory but not the reconsolidation of fear memory Jun Yin, Han Lu, Jing Ma, Qingsheng Xue, Xiaohua Cao, Fujun Zhang, Buwei Yu Department of Anesthesiology, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200025, China Corresponding author: Fujun Zhang, E-mail: fujunzhang1964@163.com 【bstract】 Objective To explore the effect of propofol on the consolidation and reconsolidation of fear memory. In the first part, the effect of propofol on the consolidation of fear memory was studied. Adult male Sprague Dawley rats were trained in a contextual fear conditioning paradigm in which they received 3 foot shocks. Different doses (100mg/kg, 150mg/kg and 200mg/kg) of propofol were administered intraperitoneally immediately after training and propofol (150mg/kg) was administered intraperitoneally immediately or 1, 2, 3h after training. The retention was performed 24h later and the open field test was conducted before the retention. In the second part, we explored the effect of propofol on the reconsolidation of fear memory. Rats were returned to the conditioning chamber for 5 min in the absence of shock 24h after contextual