基因组水平上拟南芥和水稻mrs2基因家族的比较系统发生分析.pdfVIP

中国农业科技导报，2007，9(6)：56～63 Journal of Agricultural Science and Technology 基因组水平上拟南芥和水稻mrs2基因家族的比较系统发生分析 许 花， 杨泽峰， 顾世梁 (江苏省作物遗传生理重点实验室，教育部植物功能基因组学重点实验室，扬州大学，江苏扬州225o09) 摘 要：mrs2(mitochondrial RNA splicing 2)基因是植物线粒体中Ⅱ类内含子自我剪接缺陷的抑制基因，同时 参与了植物中镁离子的运输。本研究利用已经分离的植物的mrs2基因，鉴别出MRS2结构域，同时对拟南芥 和水稻中的mrs2基因家族的成员进行了鉴定；利用这些基因编码的蛋白质序列构建了系统发生树，并进行了 序列保守性分析，最后查找了相关基因的EST表达信息。结果表明：①系统发生分析表明拟南芥和水稻的 mrs2基因的结构在拟南芥和水稻分离之前已经形成，并在分离之后按照物种特异性的方式进行了扩张；② MEME分析表明植物的Mrs2蛋白质具有高度保守的基序，并且在蛋白质中的排列顺序也大致相似；③mrs2 基因在拟南芥和水稻中的表达有差异，但在部分表达上仍保持了一致性。 关键词：拟南芥；水稻；mrs2基因家族；系统发生分析 中图分类号：Q94 文献标识码：A 文章编号：1008-0864(2007)06-0056-08 Genome-wide Comparative Phylogenetic Analysis of mrs2 Gene Family in Arabidospsis and I ce XU Hua。YANG Ze．feng。GU Shi．1iang (Key Laboratory of Crop Genetics and Physiology of Jiangsu Province，Key Laboratory of Plant Functional Genomics of Education Ministry，Yangzhou University，Jiangsu YangzhOU 225009，China) Abstract：mrs2(mitochondrial RNA splicing 2)genes were discovered as a suppressor of splicing defects in mitoehondria． They have been shown to be a transporter for the movement of Mg“ in plants．This study collected the mrs2 genes that were isolated in plants and obtained their MRS2 domains．We identified the mrs2 genes in Arabidops~and rice． A phylogenetic tree was constructed based on the Mrs2 proteins．Analyses of the conserved sequences of these pro— teins were conducted；and finally the expression information of these genes was searched．The results are as follows： (1)The phylogenetic analysis indicated that the feature of this family might have been in existence before the split of Arabidopsis and rice，and species—specific expansion might have contributed to this family after the split of Arabidopsis and