第十一章 The phototoxicity of photofrin was enhanced by PEGylated liposome in vitro.pdfVIP

Cancer Letters 241 (2006) 42–48 /locate/canlet The phototoxicity of photofrin was enhanced by PEGylated liposome in vitro Yasuyuki Sadzuka a,*, Koji Tokutomi a, Fumiaki Iwasaki a, Ikumi Sugiyama a, Toru Hirano b, Hiroyuki Konno c, Naoto Oku a, Takashi Sonobe a a School of Pharmaceutical Sciences, University of Shizuoka, 52-1 Yada, Shizuoka 422-8526, Japan b Photon Medical Research Center, Hamamatsu University School of Medicine 1-20-1 Handayama, Hamamatsu, Shizuoka 431-3192, Japan c Second Department of Surgery II, Hamamatsu University School of Medicine 1-20-1 Handayama, Hamamatsu, Shizuoka 431-3192, Japan Received 20 January 2005; received in revised form 8 September 2005; accepted 6 October 2005 Abstract In recent years, photodynamic therapy (PDT) with a photosensitizer and laser has been given attention, especially for the treatment of superficial cancers, such as lung, gastric, bladder and cervical cancer. In this study, in order to enhance the efficacy of PDT, photofrin liposome (PF-Lip) was prepared with dimyristoylphosphatidylcholine, dimyristoylphosphatidylglycerol and cholesterol. Polyethyleneglycol modified photofrin liposome (PF-PEG-Lip) was prepared by modification of PF-Lip with monomethox- ypolyethyleneglycol-2.3-dimyristoylglycerol. PF-Lip and PF-PEG-Lip entrapped with photofrin with 81.0 G5.9 and 81.2 G9.2%, respectively. The particle size of each liposome was 114.3 G5.7 nm (PF-Lip) and 118 G3.5 nm (PF-PEG-Lip), respectively. It was suggested that PEGylated liposomes has no effect on the trapping ratio of PF and particle size. Phototoxicity was enhanced by liposomalization, especially PEG-modification. However, PF-PEG