利用RAPD标记分析卧龙自然保护区不同海拔沙棘种群的遗传变异.pdfVIP

应用与环境生物学报 2007，13(6)：753～758 Chin JApplEnviron Biol=ISSN1006-687X Genetic Variation of Hippophae rhamnoides Populations at Different Altitudes in the Wolong Nature Reserve Based on RAPDs ZHAO Chunfang 一，CHEN Guojuan’一．WANG Yuhua K0RPELAINEN Helena LI Chunyang ( Chengdu Institute of Biology，Chinese Academy旷Sciences，Chengdu 6 l 004 l，China) ( Graduate Universit) of Chinese Academy of Scwnces，Beijing J 00039，China) ( Departmeal ofApplied Biology，University ofHe~inki，Helsinki FI-O0014，Finland) Abstract To estimate genetic variation and genetic structure of Hippophae rhamnoides ssp．sinensis at different ahitudes，five natural populations in the Wolong Nature Resmwe al altitudes ranging from l 800 1o 3 400 m a1)ove sea level(a．s，1．)were smweyed using random amplified polymorphic DNA markers(RAPDs)．Based on ll deeamer primers，a total of 15 1 reproduc— ible DNA loci were yielded．and 143 of them were polymorphie．accounting for 94．7％．Among the five populations investiga． 1ed，the total gene diversity( )and gene diversity within population B equaled 0，289 and 0．3 1 5，respectively，which are modest for a subspecies of H．rhamnoides that is an oulerossed and long—lived woody plant．The amount of genetic variation a— mong populations varied from 0．098 within population E(3 400 m a．s．1．)Io 0．315 within population B(2 200 m a．s．1．)． The coefficient of gene differentiation(G )among populations equaled 0．406，and revealed thai 40．6％ of the genetic vari— ance existed among populations and 59．4％ within populations．The population A f I 800 m a．s．I．)differed greatly from the oilier four populations，which contributes 1o high genetic differentiation．A UPGMA cluster analysis and principal coordinate analyses based on Nei’s genetic dista