山茱萸中马钱苷的近红外光谱法测定.pdfVIP

• 374 • 中国医药工业杂志 Chinese Journal of Pharmaceuticals 2014 , 45 (4) 山莱英中马钱昔的近红外光谱法测定 龚海燕l ，李珊2 ，谢彩侠l ，雷敬卫1 ，白雁，* (1.河南中医学院，河南郑州I 450048; 2 郑州市食品药品检验所，河南郑州 450007) 摘要:应用近红外漫反射光谱法 (NIRDRS) 快速测定山菜英中的马钱苦。扫描各样品的近红外光谱，运用偏最小二 2 乘法建立含量与近红外光谱之间的模型，测定未知样品含量。所建立的马钱背定量模型的内部交叉验证决定系数 (R ) 为 0.95895 ，校正均方根偏差 (RMSEC) 、交互验证均方根偏差 (RMSECV) 和预测均方根偏差 (RMSEP) 分别为 0.0344、 0.0625 和 0.0948。 关键词:近红外光谱法:偏最小二乘法:山菜英:马钱if ，测定 中图分类号: 0675.33 文献标志码:A 文章编号: 1001-8255 (2014) 04-0374-03 Determination ofLoganin in Fructus corni by Near Infrared Spectroscopy 2 GONG Haiyan , LI Shan , XIE Caixia , LEI Jingwei , BAI Yan* (1. Henan Universiσ ofTraditiona/ Chinese Medicine, Zhengzhou 450048; 2. Zhengzhou Institute卢r Food and Drug Con仰/， Zhengzhou 450007) ABSTRACT: A near infrared diffuse reflection spectroscopy rnethod was established for the rapid determination of loganin in Fructus corni. The NIR spectra of the powdered Fructus corni were collected , and the partial least squares 2 (PLS) was developed to correlate the spectra and the coπesponding HPLC values. The correlation coefficient (R ) of the calibration model for loganin was 0.958 95 , while root mean square eπor of calibration (RMSEC) , root mean square eπor of cross validation (RMSECV) and root mean square eπor of predication (RMSEP) were 0.034 4 , 0.062 5 and 0.094 8, respectively. Key Words: near in仕ared spectroscop