smad4基因和转化生长因子β1及其受体在乳腺癌组织中的表达及意义.pdfVIP

中国临床医学 !()()7年1 2月 第14卷 第6期 Clinical Medical Journal of China。2007．Vo1．14．No．6 923 论著 smad4基因和转化生长因子I]；1及其受体 在乳腺癌组织中的表达及意义 何冬雷 许荣华 夏立平 谈 顺 吴文J 摘要 目的：探讨乳腺癌组织中smad mRNA、转化生长因子p1(TGF p1)、转化生长因子p1受体( IGF p1R)的表达及其意 义 方法：常规石蜡包埋切片行smad mRNA原位杂交染色及TGF—p1、TGF—p1 R免疫组织化学染色。结果：在非浸润性乳 腺癌、组织学分级 1级和淋巴结无转移组织中smad mRNA、 IGF一 1、TGF p1R的阳性表达率明显高于浸润性乳腺癌、组织 学分级2级或3级和淋巴结转移纽织，差异均有显著性意义；s~lad mRNA、TGF-81和TGF p1R在乳腺癌中表达密切相关。 结论：smad mRNA、TGF—S1和TGF B1R表达可能与乳腺癌发生发展、生物学行为和预后密切相关·可作为重要的生物学标 记物。 关键词 乳腺癌； snlad mRNA； ~{-4t生长因子 1； 转化生长因子p1受体； 原位杂交技术； 免疫组织化学 中图分类号 R737．9 文献标识码 A Expression of smad4 mRNA，TGF-Iil and TGF-lil R in Breast Cancer Tissue and Its Biological Significance HE Dong l i xU Rongh ua XIA Liping TA Sul’ WU Wettchuan Department of Oncology Surgcry Department o／Patholog the A{fl iliated Hospital o{HaMan Medical College，Haikou 57()102 Abstract Objective： Io examine the expression of snlad 4 mRNA，T(iF-~I and TGF一口1R in breast cancer tissue and its biolog一 1 s_gnificance．Methods：Smad 4 mRNA was detected by in situ hybridization technique。while TGF—p1 and TGF p】R were de— teced 1)v immuno11istochemica1 method of a、 idin biotin complex in formalin fixed specimens of 72 patients．Results：The positive rate of smad RNA。TGF～31 and TGF~ql R were significantly higher in the tissues of non invasive breast cancers，histological grade 1 and witl1out lymphnode metastasis than those of invasive breast cancers，histological grade 3 and with lymphnode me tastas Conclusion：smad4 mRNA． IGF—B{．T( 卜BR{may be associated with the carcinogenesis．biological behaviors and Drognosis of breast cancer．These three factors may be used as important biological markers in breast cancer． Key Words Breast cancer； Smad4 mRNA； Transforming growth factor p1； Transforming growth factor—p1 receptor； In situ hyb