cytokeratin、vimentin、RUNX2、ERK、p-ERK、JNK、p-JNK、P38、p-P38 南京医科大学(英文版).pdfVIP

Histochem Cell Biol DOI 10.1007/s00418-011-0908-x ORIGINAL PAPER Insulin-like growth factor 1 enhances the proliferation and osteogenic differentiation of human periodontal ligament stem cells via ERK and JNK MAPK pathways Yan Yu • Jinquan Mu • Zhipeng Fan • Gang Lei • Ming Yan • Sainan Wang • Chunbo Tang • Zilu Wang • Jinhua Yu • Guangdong Zhang Accepted: 26 December 2011 Springer-Verlag 2012 Abstract Insulin-like growth factor 1 (IGF-1) is a potent treated with IGF-1, and their osteogenic capacity was mitogenic protein which can enhance the osteogenic dif- investigated in vitro and in vivo. Dimethyl-thiazol-diphe- ferentiation of periodontal ligament (PDL) fibroblasts. nyl tetrazolium bromide assay and flow cytometry results However, it remains unclear whether IGF-1 can stimulate demonstrated that 10–200 ng/mL IGF-1 can stimulate the osteogenic differentiation and osteogenesis of human the proliferation ability of PDLSCs and 100 ng/mL is the periodontal ligament stem cells (PDLSCs). In this study, optimal concentration. Exogenous IGF-1 can modify the STRO-1? PDLSCs were isolated from human PDL tissues, ultrastructure, enhance the alkaline phosphatase activity, the mineralization ability of PDLSCs, and increase the expression of osteogenic markers (runt-related transcrip- Y. Yu and J. Mu contributed equally to this work and should be considered as co-first authors. tion factor 2, osterix, and osteocalcin) at mRNA and pro- tein levels. In vivo transplantation illustrated that IGF-1