滴通鼻炎水对黏膜纤毛的毒性评价论文.docVIP

　　滴通鼻炎水对黏膜纤毛的毒性评价论文 【摘要】 目的 研究滴通鼻炎水对蛙口腔黏膜纤毛运动的影响。方法 采用蛙黏膜纤毛运动实验,用肉眼观察给药后小木屑在蛙上颚黏膜表面移动一定距离所需要的时间；用显微镜观察生理盐水组和滴通鼻炎水组对离体蛙口腔上颚黏膜纤毛持续摆动时间的影响；蛙口腔上颚黏膜用生理盐水或滴通鼻炎水浸润30 min后，分离蛙口腔上颚黏膜，用显微镜观察其纤毛持续摆动时间。结果 ①在整体动物实验中，滴通鼻炎水常用浓度、高浓度组给药前后对蛙黏膜纤毛运动均无明显影响（P 0.05）；②在离体实验中，滴通鼻炎水常用浓度、高浓度组对蛙黏膜纤毛运动亦无明显影响（P 0.05）；③在半在体法实验中，滴通鼻炎水对蛙纤毛运动持续时间有明显缩短作用（P 0.05），但作用强度显著弱于阳性对照药去氧胆酸钠。结论 滴通鼻炎水在整体实验和离体实验中对蛙黏膜纤毛的功能无明显影响，但滴通鼻炎水浸润30 min后对黏膜纤毛运动有抑制作用。 【关键词】 滴通鼻炎水；纤毛运动；毒性 Abstract:Objective To evaluate the influence of Ditong nasitis drop on frog palate mucosa cilia movement. Methods ①Record the time span of the small chips moving on the surface of frog palate mucosa by eyes; ② Observation of the time span of frog palate cilium continuing segmental motion in drug solution under microscope; ③Observation the time span of frog palate cilium continuing segmental motion after sufficient effect by drug solution under microscope. The above ine the effects of different drug concentrations on frog palate mucosa cilia movement. Result ① No apparent effect has been found on frog mucosa cilium movement at the normal dosage and high dosage (the concentration of L 1.58g crude drug/mL) of Ditong nasitis drop had dividedly acted on frog mucosa cilia, al test; ② No obvious effect had been found on frog mucosa cilial movement at the normal dosage and high dosage of Ditong nasitis drop respectively, ovement of frog mucosa cilia in vitro, indicating that Ditong nasitis drop inhibits cilial movement ucosa cilia for a relatively long time（P 0.05）. But the intensity is much cholate. Conclusion Ditong nasitis drop had no remarkable effect on injuring the function of frog mucosa cilia. Key ovement; toxicity 滴通鼻炎水由蒲公英、麻黄、黄芩、石菖蒲、苍耳子、辛夷等组成.freelL/ 瓶，广州白云山制药股份有限公司白云山外用药厂生产。质量浓度为0.395 g生药/mL，作为低剂量组用药。滴通鼻炎水浸膏（与配制上述成药同一批提取），按处方投料提取浓缩制成，广州白云山制药股份有限公司白云山外用药厂生产，质量浓度为4.266 g生药/g。试验时用生理盐水配成质量浓度为1.58 g生药/mL，为成药浓度的4 倍，作为高剂量组用药。 去氧胆酸钠，Sigma产品，批号（lot）：61H0536。试验时，用生理盐水配成ρ =1%去氧胆酸钠溶液备用。 1.2 动物 蛙80只，体质量60~80 g，雌雄兼用,粤监正字2006C081号。 2 方法与结果 2.1