英文版-Indiana_University_Purdue_University_Fort_Wayne微生物学授课讲义 lecture 12.ppt

英文版-Indiana_University_Purdue_University_Fort_Wayne微生物学授课讲义 lecture 12.ppt

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英文版-Indiana_University_Purdue_University_Fort_Wayne微生物学授课讲义 lecture 12

Enterobacteriaceae BIOL 533 Lecture 12 Enterobacteriaceae Diversity of species Ecology Found worldwide in soil, water, vegetation, and microbial flora of animals and humans Some are always associated with disease e.g., Shigella, Salmonella, Yersinia pestis Some are normal flora that can become opportunistic pathogens e.g., E. coli, K. pneumoniae, P. mirabilis Enterobacteriaceae Epidemiology Animal reservoir: most Salmonella infections Human carrier: Salmonella typhi, Shigella Endogenous spread in a susceptible patient Can involve all body sites 5% hospitalized patients develop nosocomial infections, primarily caused by Enterobacteriaceae such as Escherichia Sites of infection Microbial Physiology and Structure Cell morphology Moderate-sized Gram— rods Non-spore-forming Motile (with peritrichous flagella) or non-motile Physiology All are facultative anaerobes Simple nutritional requirements: Ferment glucose Reduce nitrates to nitrites Distinguishing Characteristics Oxidaseˉ: Distinguishes among other fermentative and non-fermentative Gram— bacilli Lactose fermentation (red colonies on MacConkey agar) Separate Escherichia, Klebsiella, Enterobacter from other lactose— Enterobacteriaceae Distinguishing Characteristics Resistance to bile salts Separate Shigella and Salmonella from normal flora in this group Eosin Methylene Blue (EMB) Lactose, eosinY, methylene blue; Lac+; grow with green sheen Virulence Factors Antigens Somatic “O” LPS Major cell wall Ag; heat stable Specific “O” antigens associated with each genus; however, cross reactions are common Salmonella and Citrobacter Escherichia and Shigella Virulence Factors Capsular K Either protein or polysaccharide Heat-labile May interfere with detection of “O” Removed by boiling organisms Capsular antigen of Salmonella typhi referred to as Vi antigen Virulence Factors Capsular K, continued Shared by different genera both inside and outside of family Cross reactions E. coli K1 with N. meningitidis and Haemophilus m

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