Simplifiedpathwaydissectionwith分析.PDFVIP

Simplified pathway diSSection with ® ® htRf phoSpho-aSSayS and cyBi - feliX liquid handling A P P L I C A T I O N N O T E ABSTRACT Higher throughput paired with reproducible and accurate results have become a “must” in these tough times of budget cuts and the ever-greater need to fight attrition. To meet this challenge, Cisbio cell signaling assay solutions and our partner CyBio instruments from Analytik Jena have combined their expertise to develop a flexible system to run Cisbio’s reliable, sensitive and rapid HTRF® phospho-protein assays on the CyBi® - FeliX liquid handling system for simplified pathway dissection. Here, the dissection of the PI3K/AKT/mTor translational control pathway is shown on different vertical signaling levels. Inhibitory effects of several compounds are measured, enabling their precise classification by elaborating a pathway map of their phospho-signatures. This application note demonstrates that Cisbio HTRF® assays and CyBio liquid handling form a perfect match offering a straightforward and flexible automated assay platform for drug discovery and cell signaling research. material and method disorders.Several known inhibitors were selected as references to analyse their corresponding phospho-