优选大孔树脂纯化甜菊苷的研究.PDFVIP

食品与药品 Food and Drug 2011年第 13卷第03期 107 优选大孔树脂纯化甜菊苷的研究 1 2 1* 李 娜 ，陈 智 ，田景振 （1. 山东中医药大学；2. 济南市第二人民医院，山东 济南 250001） 摘 要：目的 研究不同型号大孔树脂纯化甜菊苷的工艺条件及参数，为甜菊苷的工业化生产提供参考。方法 考察静态饱 和吸附量、动态吸附量及解吸的性能，以甜菊苷转移率及含量为指标，确定甜菊苷的最佳纯化工艺。结果 4种树脂中， AB-8大孔树脂吸附和解析甜菊苷的能力最好，最佳工艺条件为：上样液浓度9 mg/mL ，80 % 乙醇以3 mL/min速度洗脱， 洗脱率达90 % 以上。结论AB-8树脂综合性能较好，适于分离纯化甜菊苷。 关键词：甜菊苷；大孔树脂；纯化 中图分类号：R284.2 文献标识码：A 文章编号：1672-979X(2011)00-0107-03 Optimization of Purification of Stevioside by Macroporous Adsorption Resins 1 2 1 LI Na , CHEN Zhi , TIAN Jing-zhen (1. Shandong University of Tranditional Chinese Medicine, Jinan 250355, China; 2. The Second Peop le ’s Hosp ital of Jinan, Jinan 250001, China) Abstract: Objective To study the technological conditions and parameters for purification of stevioside with different types of macroporous resin and to provide reference for industrial production of stevioside. Methods The optimum purification technology was determined by comparing the static saturated absorptive capacity, dynamic absorptive capacity and desorption performance of different resins. Results Among 4 resins, AB-8 macroporous resin showed optimum adsorption and elution performance. The optimal technological condition was as follows: loading concentation was 9 mg•mL-1, and the column was eluted with 80 % ethanol at the rate of 3 mL/min. 90 % of stevioside could be eluted. Conclusion AB-8 showed preferable comprehensive properties and was suitable for purification of stevioside. Key Words: stevioside; macroporous resins; purification