放射性视网膜病变大鼠玻璃体和血清血管内皮生长因子的相关研究.pdf

眼科研究2OO9年 5月第 27卷第5期 391 · 实 验 研 究 · 放射性视网膜病变大 鼠玻璃体与血清血管 内皮 生长因子 的相关研究 李玉凤 周 平 Research 0n Vascular end0thelialgrOwth factOr in VitreOus and serum in radiatjOnretjnOpathyrat y吐厂e凡g，Z 0 Png．DPn e凡 Q厂I9p 0Z玎zoZ0gy， Z0edF， 日．0P 。ZQ厂Gnng edc。ZU凡e fy， Nnnning53oo2l，Chtn。 Abstract objectiVe Radiationretinopathyisaseriouscomplicationf0ll0wingtheradiati0nirradiationofoculartum0ur． Radiation retin。pathy ismainlycharacterized byne0vascularization ofretina．Thegoal0fthisstudywast0observethecontentof vascularend0thelialgrowthfactor(VEGF)inserumandvitre0us0fSpregue—Dawley(SD)ratswithradiationretinopathyratsand exploretheIle0fVEGF in thedevelop啪ent0fradiationretjnopathy． M etbods dioative—raywasusedt0jn_adiate3Oma1eSD ratswiththeabsorbingd0se0f0．8／min．Theirradiatedratswererandomlydividedint010Gyirradiationgroup(12．5min)and 30Gyirradiati0ngr0up(37．5min)．0thernormal15SD ratswereascontrolgroup．Theratsweresacrificedandserum and vitreous pecimenswerecol1ected in 3，6and9monthsafterirradiation．Retinawasdenudedfortypsin digestion and stretched preparati0n．VEGF contentsin serum andvitreousweredetectedbyELISA． Results In3OGy iⅡadiation gr0up，theVEGF concentrationsinvitreouswas(84．66±5．97)ng／mL，(316．27±9．62)ng／mLand (663．98±5．93)ng／mLin3，6and9 m0nthsafterirradiati0n，beinghigherthan thatofnormalcontrolg0up (77．04±4．36)ng／mL(P 0．01)．TheVEGF c0ncentra【ioninserumwas(5．73±2．65)ng／mL，(7．09±0．85)ng／mLand (7．16土2．61)ng／mLin3，6and9months respectively，showingainsignicantlydifferenceincomparisonwithcontrolgr0up(6．78±3．61)ng／mL (PO．05)．In1OGy irradiationrats，c0ncentrati0nofVEGFinvitreouswas(72．32±2．16)ng／mL，(75．o6±5．55)n mLand(85．o5±4．46)ng／mLin 3，6and9monthsrepectivelyandthatofno珊alcontmlgr0upwas(70．27±1．65)ng／mL，sh0wingasignificantdi咕