试卷(国外英文资料).docVIP

试卷(国外英文资料) Noun interpretation 1, isoelectric point: in a certain pH environment, the amino acids dissociate into positive ions and negative ions with the same trend, the net charge is zero, and the electric field does not swim. At this point, the pH value of the environment at which the amino acid is located is called the isoelectric point of the amino acid 2. The three stage structure of proteins: proteins that fold on a two level basis and fold into a specific globular molecular structure by means of a variety of secondary bonds 3, B-: two common protein folding structure, is composed of a peptide bond stretching and folding sheet is formed by a peptide bond between the carboxyl oxygen and located in the same peptide bond another amide oxygen hydrogen bond, hydrogen bond is almost vertical extension of the peptide bond, these peptides can it is arranged in parallel or antiparallel alignment 4. Refolding of proteins: under certain conditions, denatured protein molecules restore their original natural conformation and restore biological activity 5, dialysis: by use of small molecules through semipermeable membrane diffusion into the water (or buffer) principle, a separation and purification technology to separate small molecules and biological macromolecules. 6 ion exchange chromatography: a polymeric resin or gel column with a fixed charged group 7, allosteric effect: some proteins that do not directly involve protein activity, combine with other parts of the active site of proteins, cause conformational changes of protein molecules and lead to changes in protein activity 8, Michaelis constant: when the rate of enzyme reaction reaches the maximum, the concentration of the substrate is half of the reaction speed. The Michaelis constant is the characteristic constant of an enzyme, only related to the nature of the enzyme, and is independent of substrate concentration and enzyme concentration 9. The cofactor of the enzyme or the nonprotein part of the bound protein t