化学信息学-数据库.pptx

ACS数据库 第一篇 题目:A Highly Selective Ratiometric?Two-Photon?Fluorescent?Probe?for Human Cytochrome P450 1A 作者:Zi-Ru Dai?, Guang-Bo Ge??, Lei Feng??, Jing Ning?, Liang-Hai Hu⊥, Qiang Jin?, Dan-Dan Wang?, Xia Lv?, Tong-Yi Dou?, Jing-Nan Cui*?, and Ling Yang*? 摘要:Cytochrome P450 1A (CYP1A), one of the most important phase I drug-metabolizing enzymes in humans, plays a crucial role in the metabolic activation of procarcinogenic compounds to their ultimate carcinogens. Herein, we reported the development of a ratiometric?two-photonfluorescent?probe?NCMN?that allowed for selective and sensitive detection of CYP1A for the first time. The?probe?was designed on the basis of substrate preference of CYP1A and its high capacity for?O-dealkylation, while 1,8-naphthalimide was selected as fluorophore because of itstwo-photon?absorption properties. To achieve a highly selective?probe?for CYP1A, a series of 1,8-naphthalimide derivatives were synthesized and used to explore the potential structure–selectivity relationship, by using a panel of human CYP isoforms for selectivity screening. After screening and optimization,?NCMN?displayed the best combination of selectivity, sensitivity and ratiometric fluorescence response following CYP1A-catalyzed?O-demetylation. Furthermore, theprobe?can be used to real-time monitor the enzyme activity of CYP1A in complex biological systems, and it has the potential for rapid screening of CYP1A modulators using tissue preparation as enzyme sources.?;TOC: ;第二篇 题目:A?Two-Photon?Fluorescent?Probe?for Thiols in Live Cells and Tissues 作者:Jun Han Lee, Chang Su Lim, Yu Shun Tian, Ji Hee Han and Bong Rae Cho* 摘要:We report a?two-photon?fluorescent?probe?(ASS) that can be excited by 780 nm femtosecond pulses and detect thiols in live cells and living tissues at a 90?180 μm depth without interference from other biologically relevant species by?two-photon?microscopy.;TOC: ;RSC数据库 第一篇 题目: Recent development of two-photon fluorescent probes for bioimaging 作者:Dokyoung