青蒿琥酯对人胚肺成纤维细胞增殖影响的分子生物学机制研究.pdfVIP

关键词：青蒿琥酯；人胚肺成纤维细胞；Fas；FasL；Caspase-3 2 The studying of the mechanism on artesunate -induced apoptosis of human embryonic lung fibroblasts Abstract 1. Role of Fas, FasL in artesunate-induced apoptosis of human embryonic lung fibroblasts Objective: To explore the effect of artesunate on apoptosis in HFL-I cells and the role of Fas, FasL in artesunate-mediated apoptosis, and further provide experimental data for anti-fibrosis activity of artesunate. Methods: CCK-8 assay was used to determine the effects of artesunate on the growth of HFL-I cells in vitro. Apoptosis ratio was examined by flow cytometry (FCM). The mRNA level of Fas and FasL were assessed by RT-PCR. Results: Artesunate has a significantly inhibitory effect on the proliferation of HFL-I cells in a dose-dependentmanner in vitro. Apoptosis rate of HFL-I cells was significantly increased in artesunate-treated group compared with control group (P0.01). The mRNA levels of Fas and FasL were significantly increased in artesunate-treated group, compared with control group. Conclusion: Artesunate may exert marked anti-pulmonary fibrisis effect by upregulating mRNA level of Fas and FasL, which could induce the growth 3 inhibition and apoptosis in HFL-I cells. 2 ． Effect of artesunate on the expression of Caspase-3 in human embryonic lung fibroblasts Objective：To study the effect of artesunate on the growth of human embryonic lung fibroblast HFL-I cells in vitro and provide experimental data for anti-fibrosis activity of artesunate. Methods ：CCK-8 assay was used to determine the effects of artesunate on the growth o