黄精种质资源遗传多样性研究.pdfVIP

浙江农林大学学报. 2016 , 33(6): 1085-1091 JOU T1wl 01 Zhejiang A F University doi: 10.11833/j .issn.2095-0756.20 16.06.023 黄精种质资源遗传多样性研究 刘跃钧l 气张援l ，蒋燕锋2 ，刘京晶l (1.浙江农林大学亚热带森林培育培育国家重点实验室培育基地，浙江临安 3113∞; 2. 浙江省丽水市 林业科学研究院，浙江丽水 323仪)()) 摘要:为揭示中药材黄精种质资源的遗传多样性及种源问亲缘关系，建立了目标起始密码子多态性分子标记 (SCoT) 方法，采用非加权平均距离法(UPGMA)和主坐标分析(PCoA)等方法对 19 份不同种源的黄精Polygonatum 材 料进行分析。从待选的 29 个引物中筛选出 15 个 SCoT 引物用于扩增基因组 DNA，总共得到 500 个条带，平均每个 引物产生 33.3 个条带，其中多态性条带 498 个，多态性比率为 99.6% ， 19 个黄精种质间的遗传相似系数范围为 0.5540-0.7340。结果表明:不同种源的黄精种质有较丰富的遗传多样性， SCoT 分子标记可以为黄精的分类及鉴别 提供一定的依据，为中药黄精优良品种的培育JJ..资源可持续利用提供研究基础。图 3 表 3 参 20 关键词:中草药学;黄精; SCoT; 种质资源;聚类分析;遗传多样性 中图分类号: S759.82 文献标志码:A 文章编号: 2095-0756(2016)06-1085-07 Genetic diversity of Polygonatum germplasm 1 1 LIU Yuejun气 ZHANG Yuan , JIANG Yanfeni, LIU Jingjing ( 1.四le Nurturing Station for State Key Laboratory of Subtropica1 SilvicuIture , Zhejiang A F University , Lin an 311300, Zhejia吨， China; 2. Lishui Institute of Forestry Sciences, Lishui 323∞0， Zhejia吨， China) Abstract: To explore the genetic diversity and relationship of germplasm resources of polygonati rhizoma (de- rived from Polygonatum cyrtonema and P. sibiricum) , the Start Codon Targeted (SCoT) molecular marker tech- nique was established , and the unweighted pair-group method with arithmetic means (UPGMA) and principal coordinates analysis (PCoA) were used to study the genetic diversity of 19 Polygonatum provenances followed by a cluster analysis. Fifteen SCoT primers were eventually screened from 29 primers and then used to amplify the genome DNAs. Results showed a total of 500 SCoT loci were produced with 33.3 SCoT 10cÎ per primer