海洋活性生物碱fascaplysin对肝癌细胞BeL_7402代谢物的影响.pdfVIP

fascaplysin BeL7402 1, 3 2* 2 2 2 郑燕玲 , 严小军 , 徐继林 , 金海晓, 陈娟娟 ( 1, 266071; 2 , 315211; 3, 100049) : 考察海洋生物活性碱 fascap lysin 对肝癌细胞 Bel7402代谢物的影 利用超高效液相色谱四极杆飞行 时间质谱, 采用电喷雾电离源分别在正负离子模式下, 对通过 fascaplysin处理 412 和 24 h 的 Bel7402的乙腈提取物进行分 析, 并通过 S IMCAP软件进行主成分分析和正交偏最小二乘法辨别分析 12 h 后 fascap lysin处理组能够与对照组在 PCA得分图上完全区分开, 24 h 后两组处理则显著分开分析 fascaplysin作用 Bel7402细胞后的潜在代谢标志物, 发现主要 为磷脂酰乙醇胺磷脂酰胆碱鞘磷脂脂肪酰胺植物鞘氨醇二氢神经鞘氨醇二甲基鞘氨醇N Palm itoyl Ta rine和Noleoyl Ta rine 鞘磷脂的下调以及磷脂酰胆碱植物鞘氨醇二氢神经鞘氨醇二甲基鞘氨醇的上调表明胞内鞘磷脂途径被激 活可能是 fascaplysin 引起细胞凋亡的一种作用机制 : fascaplysin; BeL7402; 代谢标志物; 超高效液相色谱四极杆飞行时间质谱 : R965 : A : 1001- 2494( 2010) 18- 1393- 07 Effect ofM arine BioactiveA lkaloid Fascaplysin onM etabolites ofHepatocellular Carcinoma CellL ine Bel 7402 1, 3 2* 2 2 2 ZHENG Yanling , YAN X iaoj n , XU Jilin , JIN H aixiao, CHEN J anj an ( 1Institute of Oceanology, Chi neseAcademy of Sciences, Qingdao 266071, China; 2K ey Laboratory of M arine iotechnology, N ingbo University, N ingbo 315211, Ch ina; 3 Graduate University of the Ch ineseA cademy of Sciences, eijing 100049, Ch ina) AB TRACT: OBJECTIVE To st dy the effect of m arine b ioactive alkaloid fascap lysin on m etabolites of hepatocell lar carcinom a cell line B eL7402M ETHOD A ceton itrile extract from BeL7402 cellswas treated w ith fascap lysin for 4, 12, 24 h andw as st d ied by ltra perform ance liq id chrom atographyq adr poletmi e of flight mass spectrom etry ( UPLCQTOFMS) sing electron spra ying ionization w ith pos itive and negativem odePrinciple component analysis ( PCA ) and orthogonalprojection to latent str ct res d is crmi inant analysis ( OPLSDA) w ere b ilt sing the SIM CAP to separate the control treatm ent and dr g treatm ent and to find com po nds expressed in s ignificantly d ifferent amo nts betw een the tw o pop lationsRE ULT Fascap lys intreated gro p and con t