白介素18促动脉粥样硬化斑块内细胞凋亡的作用.docxVIP

白介素-18促动脉粥样硬化斑块内细胞凋亡的作用来源：创新医学网? 　　作者：李巧汶,李志梁,邱健1　　作者单位：1 广州市广州军区总医院心内科(南方医科大学珠江医院心内科，广东广州 510282)　　【摘要】目的探索白介素-18(IL-18)促进动脉粥样硬化斑块内细胞凋亡的作用及其机制。方法采用30只老年同龄雄性动脉粥样硬化模型新西兰大白兔制成60个动脉粥样硬化板块标本，采用析因设计，给予IL-18进行刺激，以不同的IL-18刺激时间和刺激浓度组合作为新处理组，将60个动脉粥样硬化斑块标本随机分入新处理组中(n=5)，测量在不同刺激时间和刺激浓度下，纤维帽的厚度与脂核横截面厚度的变化，TUNEL法检测斑块内细胞凋亡的改变。结果帽/核比值与斑块内细胞凋亡指数随着IL-18的浓度及刺激天数的改变而变化(P0.01)。帽/核比值随着IL-18的浓度及刺激天数增加而降低(P0.01)，斑块内细胞凋亡指数随着IL-18的浓度及刺激天数增加而升高(P0.01);斑块内细胞的凋亡指数与帽/核比值呈显着线性负相关(r=-0.776，P0.001)。结论 IL-18诱导斑块内细胞凋亡可能是其促易损斑块形成的重要机制。　　【关键词】 IL-18;动脉粥样硬化;细胞凋亡;易损斑块　　【Abstract】 Objective To explore the role and mechanism of interleukin-18 (IL-18) in promoting apoptosis of cells in atherosclerotic plaques.Methods 30 male New Zealand white rabbits were selected and euthanized.The entire length of the aorta was exposed and divided into several segments.Histological assessment of experimental atherosclerosis was evaluated.Rabbits in experimental group were received stimulation of IL-18.New group was established according to work time and concentration of IL-18，the 60 specimens were randomly divided into the new treatment group (n= 5)，the thickness of fibrous cap and nuclear cross section were measured after stimulation of IL-18.At last，apoptosis was detected by TUNEL.Results The cap/core ratio and apoptotic index of plaques were changed according to the concentration and work time of IL-18|(P0.01)，with the concentration and work time of IL-18 increased，the cap/core ratio of plaques reduced (P0.01) and apoptotic index of plaques raised inversely (P0.01)，apoptotic index of cell in plaques was significantly negatively correlated with the cap/core ratio(r=-0.776，P0.001).Conclusions IL-18 inducing apoptosis in plaque may be the main mechanisms of promoting the formation of vulnerable plaque.　　【Key words】 Interleukin-18;Atherosclerosis;Apoptosis;Vulnerable plaque　　目前认为，易损斑块(VP)溃疡、破裂、血栓形成是急性冠脉综合征(ACS)发生的主要病理生理过程。炎症是VP形成的最重要的机制之一，白介素-18(IL-18)作为在炎症反应系统中发挥中心性作用的炎症因子，不但与动脉粥样硬化(AS)斑块的发生、发展〔1，2〕，且参可能与VP的形成〔1，3〕，但IL-