VEGF在胰腺缺血再灌注损伤中的作用.docxVIP

VEGF在胰腺缺血再灌注损伤中的作用来源：创新医学网　　作者：汤礼贵,宋胜江,安慧敏　　作者单位：1.瑞安市人民医院，浙江 温州 325200;2.温州医学院，浙江 温州 　　【摘要】 目的 ：探讨血管内皮生长因子(VEGF)在缺血再灌注损伤(IRI)胰腺组织中的表达及其意义。方法：将雄性SD大鼠32只随机分为4组(n =8)。A组为对照组;B组、C组和D组分别通过钳闭大鼠腹腔干和肠系膜上动脉15、30和60 min造成胰腺缺血，然后再灌注6 h建立缺血再灌注损伤模型。对各组胰腺组织进行湿干质量比(W/D)检测、病理损伤评估及VEGF免疫组化染色。结果：经缺血再灌注后，胰腺组织的VEGF蛋白表达增强，VEGF蛋白表达在胰腺的胰岛细胞、间质血管内皮细胞、腺泡及导管上皮细胞之间差异有显著性(P0.01)。胰腺病理评分和W/D比值与胰岛细胞的VEGF免疫组化染色评分呈正相关(P0.01)，与间质血管内皮细胞的VEGF免??组化染色评分呈高度正相关(P0.01)。结论：胰腺IRI引起的胰腺水肿可能与VEGF表达增强有关。 　　【关键词】 血管内皮生长因子;缺血再灌注损伤;胰腺 　　Role of vascular endothelial growth factor in pancreatic ischemia reperfusion injury TANG Li-gui*， SONG Sheng-jiang， AN Hui-min， CAI Xiao-fang， ZHU Hua. *Department of Surgery， Rui’an People’s Hospital， Wenzhou， 325200 　　Abstract: Objective: To explore the expression and significance of vascular endothelial growth factor (VEGF) in ischemia reperfusion injury (IRI) of the pancreas. Methods: Thirty two male SD rats were randomly divided into four groups (n =8). Group A served as control group， Groups B， C and D were subjected 15 mins， 30 mins and 60 mins of ischemia respectively by clamping of celiac artery and superior mesenteric artery， then released for 6 hours to produce ischemia reperfusion injury model. Rats were sacrificed and the pancreas was removed for histological injury evaluation and detection of wet/dry weight ratio and immunohistochemical staining of VEGF. Results: The expression of VEGF in pancreas increased after ischemia reperfusion injury. There was differential expression of VEGF among the islet cells，stromal vascular epithelial cells，acinar and duct epithelial cells of pancreas (P0.01). Pathological score and wet/dry weight ratio of the pancreas were correlated positively with the score of immunohistochemical staining of VEGF in the islet cells (P0.01) and showed a high positive correlation with that in stromal vascular epithelial cells (P0.01). Conclusion: The edema of the pancreas caused by ischemia reperfusion injury may be related to the increased expre