碘125巨细胞刺激因子测定法的建立.pdfVIP

— 26 — Chin J Lab Diagn ,February ,2002 ,Vol 6 ,No . 1 ( ) 文章编号 :1007 - 4287 2002 06 - 0026 - 03 125 　I - 巨噬细胞集落刺激因子测定法的建立 1 2 1 1 3 许树旭 ,蒋少华 ,夏瑞雪 ,李卫杰 ,高 　琪 ( 1 北京军区266 医院 ,河北 承德 067000 ;2 北京福瑞生物工程公司 ;3 北京邦定生物技术公司) 摘要 : 目的　通过检测卵巢肿瘤患者血清 ,从而辅助临床早期作出肿瘤 良、恶性诊断 。方法 　在表达及分离纯化 ( ) 巨噬细集落刺激因子 MCSF 的基础上 ,成功地制备了MCSF 抗体 , 以夹心法测定 MCSF 。结果 　MCSF 测定范围为 05～200ng/ ml ,灵敏度 01788ng/ ml ,批内及批间变异 10 % , 回收率平均为 1022 % 。66 例正常人血清测定范围在 102～210ng/ ml ,应用本法 46 例卵巢癌患者血清测定值范围在 25～82ng/ ml ,卵巢 良性肿瘤均低于 25ng/ ml 。结论 　该法具有简便 、快速和准确的特点 ,适于临床检测和科研应用 。 关键词 :MCSF ;卵巢肿瘤 ; 中图分类号 :R44611 　　　　　　　文献标识码 :A Radioimmunoassay of Macrophage Colony - Stimulating Factor in overian cancer X U S hux u , J IAN G S hao - hua , XIA Rui - ( ) x ue , et al . B eij ing Command 266 th Hosp ital , He B ei Cheng De , 067000 Abstract :Objective 　To detect serum level of hMCSF for diagnosis patients are or not ovarian cancer . Methods Based on isolation ,purification of MCSF ,Successfully preared anti - MCSF antibody and developed a radioimmunoassay for MCSF. Results Detectable range of MCSF is between 05～20ng/ ml . The sensitivity or minimal detectable concentration dose was 01788ng/ ml . The between - runs CVb and the within - runs of 3 samples was lower than 8. 0 % and 3 . 0 % respectively. The average concentration of MCSF in sera from 66 healthy women was 1. 58 ±0 . 50ng/ ml ( 1. 02 ～10ng) . 46 ovarian cancer patients in 25 ～8. 2ng/ ml . Conclusion The assay is a rapid ,sensitive ,precise method and simple to perform