三维重组人工皮肤模型在化妆品安全性评价中的应用.docVIP

三维重组人工皮肤模型在化妆品安全性评价中的应用 孔雪 聂鹏举 何文丹 唐颖* （中国化妆品创新协同中心/北京市植物资源研究开发重点实验室/北京工商大学理学院化妆品系，北京100048） 摘要: 化妆品安全性评价是对化妆品进行从原料选用到准投放市场产品的潜在不良反应进行定性和定量的毒理学评价。传统的化妆品安全性评价主要采用动物试验，随着科学技术的发展和3R（减少、替代和优化）原则的推动，三维重组人工皮肤模型因具有与人类皮肤相似的生理结构和代谢功能而在的毒理学研究中获得了广泛的发展。本文主要综述了三维重组人工皮肤模型在化妆品皮肤刺激/腐蚀性评价、眼刺激性评价、光毒性评价和遗传毒性评价方面的应用现状，分析了皮肤模型的优缺点，并对其在化妆品安全性评价中的应用前景以及发展策略提出了意见和建议。 关键词：三维重组人工皮肤模型；化妆品安全；体外毒理学；动物替代方法 Safety Evaluation of Cosmetics based on In Vitro three-dimensional reconstructed human epidermis（3D-RHE）models KONG Xue, NIE Peng-Ju, HE Wen-Dan, TANG Ying (China Cosmetic Collaborative Innovation Center/Beijing Key Lab of Plant Resource Research and Development/Department of cosmetics, School of Science, Beijing Technology and Business University, Beijing 100048,China Abstract: Cosmetics safety evaluation is employing a series of toxicological tests, on both qualitative and quantative levels, to assess the potential risks for the daily use of selected cosmetic ingredients and final products. Traditional safety evaluation is mainly using animal tests. With the development of in vitro science and 3Rs (Reduction, Replacement and Refinement) principle, three-dimensional reconstructed human epidermis models have been developed and applied in cosmetic safety evaluation. Reconstructed human skin models possess anatomy and metabolism biology similar to real human. This paper reviews the current status of three-dimensional reconstructed human epidermis models applied in skin irritation/corrosion, eye irritation, photo-toxicity and genotoxicity assessment of cosmetics. The advantages and disadvantages of using skin models are also discussed with comments and suggestions for its future development. Keywords: three-dimensional reconstructed human epidermis models; cosmetic safety; in vitro toxicity; animal alternative methods  近年来化妆品行业问题频发，化妆品的安全性日益受到企业和消费者的关注。化妆品的安全性评价是对化妆品潜在不良作用进行定性和定量的认定和评价，目的在于降低化妆品不良作用、预测化妆品的远期效应以及制订安全接触限值[1]。传统的安全性评价主要采用动物模型进行。然而，动物试验存在评价周期较长、重复性差、精确度低等缺点，而且动物与人具有种间的生