γ射线对白血病细胞株K562中MMP-2表达的影响及牛磺酸的防护作用.docVIP

276 Chin J Hemorh. 2002 ;12(4) γ射线对白血病细胞株 K562 中 MMP - 2 表达的影响 Ξ 及牛磺酸的防护作用 范 　雁 　吴士良 　徐 　岚 　仇 　灏 　徐爱华 (苏州大学生化工程研究所 ;苏州大学医学院生化及分子生物学教研室 ,苏州 　215007) 摘要 :目的 　探讨γ射线对白血病细胞中 MMP - 2 表达的影响 ,以及从细胞水平初步探讨牛磺酸对肿瘤细胞受 辐照后 MMP - 2 表达的抑制作用。方法 　60Coγ射线 15Gy 照射白血病细胞 K562 作为阳性对照组 ,不照射的作为阴 性对照组(1 - 不加药 ,2 - 加药 200mg/ L) ,另设三组实验组(照射 ,加药量分别为 50mg/ L、100mg/ L、200mg/ L) ,照后 12h 收集 ,细胞经处理后采用 Western2blotting 技术分析各组细胞 MMP - 2 的表达水平。结果 　15Gy 照射剂量阳性对照组 细胞 MMP - 2 表达量明显增加 ,阴性对照组 1 中 MMP - 2 表达量较少 ,实验组细胞 MMP - 2 表达量随剂量增加而依次 减少 ,其中 200mg/ L 组与阴性对照组 1 细胞 MMP - 2 表达量基本一致。结论 　15Gy60 Coγ射线照射促进白血病细胞 K562 中 MMP - 2 表达 ,电离辐射可引起肿瘤细胞的进一步侵袭和转移 ;加不同剂量牛磺酸后对 MMP - 2 表达有抑制 作用 ,且与剂量呈相关性 ,实现生物防治的作用。 关键词 :γ射线 ;牛磺酸 ;白血病细胞 K562 ;MMP - 2 (Matrix Metalloproteinase) 中图法分类号 :R815 　　文献标识码 :A 　　　　文章编号 (2002) 04 - 0276 - 03 Effect ofγ2rays on the Expressions of MMP - 2 in K562 Leukemia Cell Line and the Biologic Protection of Taurine FAN Yan , WU Shi2liang , XU Lan , et al. (Suzhou University ,Department of Biochemistry and Molecular ,Suzhou 215007 ,China) Abstract :Objective 　To study the effect ofγ- irradiation on expressions of MMP - 2 to Leukemia cells and Tau’s inhibition to the expressions of MMP - 2 of the irradiated tumour cells in term of cellular level. Meth2 ods 　The cells in the control group and Tau (50mg/ L. 100mg/ L. 200mg/ L) groups were irradiated with 15Gyγ - rays. The expressions of MMP - 2 were examined through Western - blotting within 12h. Results 　The ex2 pressions of MMP - 2 were enhanced evidently in the positive control group , while they were less in the negative control group ; In the Tau(50mg/ L. 100mg/ L. 200mg/ L) groups , the expressions of MMP - 2 were diminished in turns , and they were almost identical within negative control group and the Tau (200mg/ L) group. Conclu2 sion 　Radiation withγ- rays at a dose of 15Gy can significantly stimulate the expressions of MMP - 2 in K562 cells ,and it can causes more invasiveness and migration of tumour cells ;Tau can inhibit the expressi