1株广谱拮抗菌的分离鉴定及其抗菌活性成分分析.PDFVIP

() 2014 10 40 5 Journal of Hunan Agricultural University (Natural Sciences), Oct. 2014, 40(5):513–518 DOI:10.13331/ki.jhau.2014.05.011 /qks 1 株广谱拮抗菌的分离鉴定及其抗菌活性成分分析 1,2 1,2 1.2 珺1.2 1,2 1,2,3* (1. 410009 2. 410009 3. 410128) 摘 要––() 1 HMI–23 16S rDNA (Bacillus amyloliquefaciens)K ABDB 关 键 词 中图分类号S432.4 文献标志码：A 文章编号：1007− 1032(2014)05−0513−06 Isolation and identification of a broad spectrum antagonistic bacteria and its antibiotic component analysis GUO Zhao-hui1,2 , HUANG Jun1,2WEI Xiao-wu1,2, LUO Rong-jun1,2, WU Shan-dong1,2, LIU Qing-shu1,2,3* (1.Hunan Institute of Microbiology, Changsha,410009, China; 2.Agricultural Microbiology Application Engineering and Technology Research Center of Hunan Province, Changsha, 410009, China; 3.College of Plant Protection ，Hunan Agricultural University, Changsha 410128, China) Abstract: A strain of Bacillus sp. named HMI-23, which has been identified as a member of Bacillus amyloliquefaciens by 16S rDNA analysis, and exhibited extensive antagonistic ability on Rhizoctonia solani ，Sclerotinia sclerotiorum ， Ralstonia solanacearum was isolated from tobacco-rice-rape rotation soil. The inhibition ability of HMI-23 fermentation broth supernatant was insensitive to proteinase K, ultraviolet ray and high temperature. Four kinds of lipopeptides synthesis related genes have been detected in the genome of strain HMI-23 by PCR. The lipopeptides crude extract of HMI-23, which may contain several known lipopeptide including iturin A, mycosubtilin B, bacillomycin D, and fengycin B by LC/MS analysis, have strong abi