中药莪术对大鼠十二指肠平滑肌收缩的影响.PDFVIP

中药莪术对大鼠十二指肠平滑肌收缩的影响 唐泽耀1 1 2 1 1* ，王薇薇 ，沈德利 ，傅雷 ，林原 (1.大连医科大学药理教研室，辽宁 大连 116044 ；2.大连市第三人民医院药剂科， 辽宁 大连 116033) 摘要：目的 探讨中药莪术(Curcumna Rhizoma)水提液对大鼠离体十二指肠平滑肌收缩运动的影响，并初步探索其作用 机制。方法 制作大鼠离体十二指肠标本，用恒温灌流的方法，使用BL-420 生物机能实验系统观察不同浓度莪术对大鼠 离体十二指肠平滑肌的收缩效应。选适当终浓度莪术分别与阿托品、去甲肾上腺素、异丙肾上腺素、维拉帕米共同孵育， 观察莪术对它们引起平滑肌收缩作用的影响；制作不同浓度新斯的明肠管收缩作用的量效曲线，与莪术组进行对比，初 步探讨其可能的作用机制，并作胆碱酯酶活性测定辅助证明是否与新斯的明作用机制相似。结果 莪术可以对大鼠离体 十二指肠的收缩有兴奋作用，呈剂量依赖性；莪术可以部分被M 受体阻断药阿托品阻断、β受体激动药异丙肾上腺素和 钙通道阻滞剂维拉帕米对肠管收缩的抑制作用；对 α 受体激动药去甲肾上腺素无影响；与乙酰胆碱酯酶抑制剂新斯的明 的作用相似。结论 莪术明显促进大鼠离体十二指肠平滑肌收缩作用，呈剂量依赖性。其作用机制可能是通过抑制胆碱 酯酶，与M 受体介导有关，同时还可能与抑制α和β受体有关。 关键词：莪术；十二指肠；促进；机制 中图分类号：R284.1 文献标志码：A 文章编号：1007-7693(2012)09-0772-06 Effect of Curcumae Rhizoma on Rat Duodenum Smooth Muscle Contraction 1 1 2 1 1* TANG Zeyao , WANG Weiwei , SHEN Deli , FU Lei , LIN Yuan (1.Department of Pharmacology, Dalian Medical University, Dalian 116044, China; 2.Department of Pharmacy, Dalian Third Peoples Hospital, Dalian 116033, China) ABSTRACT: OBJECTIVE To investigate the effect Curcuma Rhizoma on rat duodenum smooth muscle contraction and its mechanisms. METHODS After isolated rat duodenum samples were prepared, it was observed that the effect of Curcuma Rhizoma at different concentrations on rat duodenum smooth muscle contraction using BL-420 biological and functional experimental system with constant perfusion way. It was observed that the effect of Curcuma Rhizoma at different optimum concentrations on smooth muscle contractile amplitudes induced by atropine, norepinephrine, isoproterenol, verapamil respectively. Dose-effect curve of different concentrations of neostigmine on intestinal contraction was recorded, comparing with that of Curcuma Rhizoma group. Cholinesterase activi