32 767-771 食品安全与检测 5-10 保健食品中真菌多糖测定方法的比较.pdfVIP

现代食品科技 Modern Food Science and Technology 2010, Vol.26, No.7 真菌保健食品中多糖含量测定方法的比较 1,2 1 1 黄生权 ，敖 ，郭爱玲 （1.无限极 (中国)有限公司，广东江门 529156 ）（2.华南理工大学轻工与食品学院，广东广州 510640 ） 摘要：采用不同的测定方法测定真菌保健食品中的粗多糖的含量，考察标准品、提取方法、醇沉时间及显色方法等因素 对多糖测定结果的影响。实验表明选用硫酸苯酚法进行显色，葡萄糖做标准品，超声功率250 W ，超声提取30 min ，醇沉10 min ，测定结果较好。该方法精密度、稳定性好，平均加样回收率高，方法简单，快速，准确，可用于真菌保健食品各种粗多 糖的测定和质量控制。 关键词：灵芝；保健食品；多糖；测定方法 文章篇号：1673-9078(2010)7-767-771 Comparison of Methods for Determination of Polysaccharides Content in Epiphyte Health Foods 1,2 1 1 HUANG Sheng-quan , AO Hong , GUO Ai-ling (1.Infinitus (China) Co., Ltd., Jiangmen 529156, China) (2.College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, China) Abstract: The contents of crude polysaccharides in epiphyte health products were determined by different methods. And the effects of standard sample, extraction method, alcohol precipitation time and coloration method on the determined values of polysaccharide contents were studied. The phenol-sulphate acid method showed to be the best coloration method using glucose as standards. And the best extraction conditions were determined as follows: ultrasonic power 250 W, extraction time 30 min and alcohol- precipitation time 10 min. This method was simple, rapid, accurate and reproducible, which can be used to determine content of polysaccharides in epiphyte health product. . Keywords: Ganoderma lucidum; health food; polysaccharides; determination method 767 [1~3] 多糖含量的测