预热适应成纤维细胞NIH-3T3应激保护相关蛋白的初步筛选.pdfVIP

中国病理生理杂志 Chinese Joumal of Pa由。Ihysiol~ 2侃)6，22(8) :1493 -1496 , 1493. [文章编号] 1【削 -4718(2∞6)08 -1493-04 预热适应成纤维细胞 NIH -3T3 应激 保护相关蛋白的初步筛选* 陈雪梅，邹飞A (南方医科大学劳动与职业卫生学系，广东广州 510515) {摘 要] 目的:初步分析应激与适应对蛋白质合成的影响，筛选应激适应相关蛋白。方法:通过双向电泳分 离总蛋白、PDQUEST 软件分析、肤质量指纹图谱分析候选蛋白肤段组成，分析应激与适应对细胞蛋白质合成的影响。 初步鉴定应激适应蛋白质点的类别。结果:预适应后再次热应激组表达增加的蛋白质点在各分子量范围均有分布， 而直接热应激组表达增加的蛋白质点大多局限于低分子量范围。肤质量指纹图谱分析候选蛋白序列，初步鉴定了 T山ulinß 、中间丝 vimentin 、起始因子eIF -4AI、 Eno1 蛋白与应激适应密切相关。结论:细胞在紧急应激状态，可能会 倾向于选择性地合成能够迅速翻译的小分子量蛋白质，应对应激损伤。预适应可通过提高细胞内保护性蛋白的贮 备量，在再次应激时发挥保护作用。 Tuhulinß、中间丝 vimentin、起始因子 eIF -4AI 、 Eno1 蛋白差异表达，提示细胞骨 架、蛋白质合成、糖代谢通路在应激适应中的重要作用。 [关键词] 热预处理;应激;蛋白质组;成纤维细胞 [中固分类号] R363 [文献标识码] A Primary screening of stress protective proteins in heat - preconditioned fi- broblast NIH - 3T3 CHEN Xue - mei , ZOU Fei (Departrrumt 01 Occ叩ational Hygiene , 50毗m Medical Univers町， Gua咿也ou 510515 , China) [ ABSTRACT] AIM: To establish stress adaptation model of mouse fibroblast cell line NIH - 3白 to analyze the effect of stress and adaptation on protein synthesis , and to screen for stress adaptation related proteins. 岛皿Z回ODS: A stress - adapted cell model was estahlished by thermal preconditioning (42 t: , 20 min). Total cytolytes were separated by 2 - DE , analyzed by PDQUEST software , and the selected differential expression spots we陀 detected by MOLDI - TOF. The effect of stress and adaptation on protein synthesis wωstudied. 响le s悦ss adaptation related spots were identified by PMF. RFSULTS: Comparative proteomics method by 2 - DE was used to find different distributions of total proteins of heat stress group and thermal pre∞nditioning group. Expression - inc