UPA和UPA-R在侵袭性垂体腺瘤中的表达及意义-第三军医大学学报.doc

UPA、UPA-R、PAI-1在侵袭性垂体腺瘤中的表达及意义 唐茂源，霍 钢*，冯清林，杨 刚 第一作者:唐茂源 汉族 在读硕士研究生 研究方向颅内肿瘤的侵袭性研究 第一作者电话E-mail：tmy840330@163.com 单位：重庆医科大学附属第一医院神经外科 通讯作者：霍钢 移动电话E-mail：xiaomin198171@ 邮编：400016 【摘要】目的 研究uPA、uPAR及PAI-1在非侵袭性与侵袭性垂体腺瘤中的表达，探讨其与垂体腺瘤侵袭性的关系。方法 61例垂体腺瘤分为侵袭性和非侵袭性两组，采用免疫荧光及Western blot检测uPA、uPAR及PAI-1的蛋白表达情况，应用RT-PCR检测uPA、uPAR及PAI-1的mRNA表达水平，并用t检验、X2检验，spearman相关分析法分析比较两组肿瘤之间uPA、uPAR及PAI-1的表达差异性。结果 在所检测的肿瘤中，31例为侵袭性垂体腺瘤，30例为非侵袭性垂体腺瘤，免疫荧光结果显示在侵袭性垂体腺瘤及非侵袭垂体腺瘤的uPA阳性表达率分别为67.74%和36.67%、uPAR为58.06%和33.33%、PAI-1为54.84%和26.67%，PCR检测结果显示侵袭性垂体腺瘤组与非侵袭组中uPA的相对表达量为0.5468±0.0464和0.4462±0.0305、uPAR为0.6463±0.0314和0.5464±0.0471，及PAI-1为0.5677±0.0577和0.4934±0.0412。Western blot侵袭组与非侵袭组的检测结果uPA为1.6260±0.1083和1.4345±0.0788，uPAR为1.7273±0.1292和1.4322±0.1214，PAI-1为1.5225±0.1216和1.2790±0.0798。检测结果显示侵袭性垂体腺瘤组中uPA、uPAR及PAI-1的mRNA及蛋白表达水平明显高于非侵袭组，并且每两个指标间存在正相关性，结果具有统计学意义。结论uPA、uPAR及PAI-1在侵袭性垂体腺瘤中的表达明显上调，说明uPA、uPAR及PAI-1的上调在肿瘤的侵袭过程中发挥着重要的作用，提示此系统可能在垂体腺瘤的侵袭过程中发挥着促进肿瘤侵袭的作用，也为后续的研究奠定了相应的基础。 【关键词】垂体腺瘤，uPA，uPA-R，PAI-1，侵袭 uPA、uPAR、PAI-1 mRNA and proteins expression and their significance in invasive Pituitary Adenomas Tang mao-yuan ,Huo Gang﹡, Feng Qing-Ling,Yang Gang (Department of Neurosurgery, First Affiliated Hospital，Chongqing Medical University，Choning,400016,China) 【Abstract】Objective：To study the expression of the genes uPA,uPAR and PAI-1 in pituitary adenomas and investigate the relationship with invasiveness of pituitary adenomas.Methods:61 speciments were classified into invasive groups and non-invasive group. The protein expressions of uPA,uPAR and PAI-1were detected by immunofluorescence and western blot analysis and their mRNA expressions were detected by reverse transcription polymerase chain reaction(RT-PCR) in all speciments.And we use t test，X2 test and spearman correlation analysis to analysis the different expression of uPA、uPA-R and PAI-1 in two group tumors.Results:31 speciments were invasive pituitary adenomas and 30 spaeciments were