一株还原制备纳米银的细菌菌株zxw01的分离鉴定.PDF

() 2015 8 41 4 Journal of Hunan Agricultural University (Natural Sciences), Aug. 2015, 41(4):423–427 DOI:10.13331/ki.jhau.2015.04.016 一株还原制备纳米银的细菌菌株 zxw01 的分离鉴定 1 1 1 1 1* 2* (1. 1500402. 150080)   摘 要1 zxw01 99%(Bacillus amyqueciens)X zxw01 218 nm 80.6% 关 键 词 中图分类号S154.34 文献标志码：A 文章编号：1007− 1032(2015)04−0423−05 Isolation and identification of zxw01, a bacteria strain with the ability to prepare silver nanoparticles via reduction 1 1 1 1 1* 2* Zhang Jie Xiong Wen Zhang Ying Wang Jiao Yang Hongyi Wang Binsong (1.College of Life Sciences, Northeast Forestry University, Harbin 150040, China; 2.School of Chemistry and Material Sciences, Heilongjiang University, Harbin 150080, China) Abstract: This research was on the acclimation and isolation of a bacteria strain ZXW01 with the ability to synthetize silver nanoparticles via reducing silver ion in its gradient of nitrate concentration. Based on morphologic observation, physiology and biochemistry tests and 16S rDNA analysis, the results proved that the bacteria was close related to Bacillus amyloliquefaciens with 99% similarity in its 16S rDNA sequence from the point of phylogenetics. Silver nanoparticles biosynthesized by the bacteria were spherical and equally distributed with the size between 2 nm to 18 nm from the analysis of X–ray diffraction, transmission electron microscopy (TEM) and thermogravimetric, and the productivity of silver nanoparticles was 80.6%. Keywords: silver nanoparticles; Bacillus amyloliquefaciens; biological synthesis