Bacillus细菌的分离及其芽孢漆酶对染料脱色的影响.PDFVIP

() 2016, 42(1):85–90. DOI:10. 13331/ki.jhau.2016.01.017 Journal of Hunan Agricultural University (Natural Sciences) Bacillus 细菌的分离及其芽孢漆酶对染料脱色的影响 ( 150040) 摘 要 1 16S rDNA Bacillus sp. 5`MS 56.73 U/g5`MS pH 6.670 ℃100 ℃ 34.61%5`MS 1 h 92%4 h 82.8% 关 键 词 中图分类号X172 文献标志码：A 文章编号：1007− 1032(2016)01−0085−06 Isolation of strain Bacillus and the effects of its spore laccase on dye decoloration and degradation Wang Chunlei, Li Fanshu, Sun Haiqiong, Zhao Min, Su Qiuyu, Qi Xuhui (College of Life Sciences, Northeast Forestry University, Heilongjiang 150040, China) Abstract: Enrichment medium added with copper ions was used to screen and isolate bacteria with laccase activity from soil at Quercus mongolica forest in northeast forestry university. The strain was identified as Bacillus sp. 5`MS judged from morphological, physiological and biochemical characteristics as well as from 16S rDNA sequence analysis. Its spore laccase activity was 56.73 U/g calculated by dry weight in syringaldazine as substrate. The optimum pH for its activity was 6.6 and the optimum reaction temperature was 70 ℃. Even though the temperature was enhanced to 100 ℃, the spore laccase activity of strain 5`MS could remain 34.61% of that in the optimal condition. The spore laccase could decolorize more than 92% of reactive black 5(RB5), indigo carmine (IC) and crystal violet (CV) in 1 hourand 82.8% of reactive brilliant blue R in 4 hours at the condition of acetosyringone as the mediator. Keywords: Bacillus ; spore laccase; dye decolorization (benzenediol: oxygen oxidoreductases, EC1. 10.3.2) [7–8] [1] [9] [2] 10 [3–4] (80 t[10]) [4]