响应面分析法优化副溶血性弧菌生长条件.PDF

微生物学通报 FEB 20, 2008, 35(2) 306~310 Microbiology ©2008 by Institute of Microbiology, CAS tongbao@ 生物实验室 响应面分析法优化副溶血性弧菌生长条件 1 1* 2 刘代新 宁喜斌 张继伦 (1. 200090) (2. 201202) 摘 要: 通过单因素分析, 确定最适于副溶血性弧菌 VPJ33 生长的 pH 、温度和盐度。在此基础上, 综合考虑 3 个因素对 VPJ33 生长的影响, 用 Design-Expert 软件进行响应面分析, 优化 VPJ33 的培 养条件得到了菌体生长模型, 以及取得模型最优值时各因素的水平。结果表明, VPJ33 的最优培养 条件为：pH 8.41 、温度 34.1 ℃和盐度 2.47 ％; 菌体生长过程中, pH 和盐度以及 pH 和温度对 VPJ33 生长的交互作用显著, 盐度和温度对 VPJ33 生长的交互作用不显著。菌体生长模型达到显著水平, 可以对 VPJ33 在不同条件下的生长情况进行分析和预测。 关键词: 副溶血性弧菌, 响应面, 生长条件 Optimization of Growth Condition of Vibrio parahaemolyticus via Response Surface Methodology LIU Dai-Xin1 NING Xi-Bin1* ZHANG Ji-Lun2 (1. College of Food Science, Shanghai Fisheries University, Shanghai 200090) (2. Shanghai Entry-exit Inspection and Quarantine Bureau, Shanghai 201202) Abstract: In this paper, the optimum pH, temperature and salinity for VpJ33 growth were determined through singe-factor analysis respectively and the Response Surface Methodology (RSM). The multivariate non-linear model was applied to study the interactions and optimization of the growth parameters for VpJ33 which were explored by experiments. The experimental results showed that the optimum conditions for ger- mination as follows: pH 8.41, temperature 34.1 ℃ and salinity 2.47 ％. Significant interactions between pH and salinity, as well as pH and temperature were found during the germination respec