树脂原位吸附提高酒明串珠菌不对称合成（R）-2-辛醇的催化效率.pdfVIP

学兔兔 化 工 进 展 2010年第29卷第2期 CHEMICAL INDUSTRY AND ENGINEERING PROGRESS ·3l9· 树脂原位吸附提高酒明串珠菌不对称合成 )．2．辛醇的催化效率 孟凡涛，徐 岩 (江南大学生物工程学院，教育部工业微生物重点实验室，江苏 无锡 214122) 摘 要：通过运用原位吸附技术构建水／树脂反应系统，利用树脂的原位吸附来提高酒明串珠菌 Oenococcus oeni CECT4730不对称合成(尺)一2一辛醇的催化效率。通过比较6种不同型号的吸附树脂对反应的影响，选出了最适的树 脂 AB一8。通过考察树脂的用量及水，树脂相中底物浓度对反应的影响，发现反应体系中加入一定量合适的吸附树 脂，能够显著减小底物和产物的抑制，大幅度的提高初始底物浓度和反应产率。AB一8树脂加量为15 g／L的水，树 脂系统中，底物浓度为30 g／L时，反应产率达到58％，较之水相系统提高了1．7倍 关键词：吸附树脂；原位分离；酒明串珠菌；不对称还原； )一2一辛醇 中图分类号：Q 814 文献标识码：A 文章编号：1000—6613(2010)02—0319—04 Enhancement of efficiency of asymmetric synthesis of )·2-octanol with Oenococcus oeni by in situ absorption of resins MENG Fnnta0，XUYan (Key Laboratory of Industrial Biotechnology of Ministry of Education，Jiangnan University，Wuxi 214122，Jiangsu，China) Abstract：To enhance efficiency of asymmetric synthesis of )一2一octanonol with Oenococcus oeni CECT 4730，we applied in situ absorption technique to compose an aqueous／resin system for the reaction．The most suitable resin AB一8 was selected from 6 types of absorption resins with respect to the effect on the bioreduction．After evaluated effect of the load of resin and the substrate concentration in the aqueous／resin system，we found that a suitable aqueous／resin system with a matched load of resin Can relieve substrate and product inhibition significantly and elevated the initial substrate concen~afion and product yield markedly．In an aqueous／resin system of 15 g／L resin AB-8，product yield could reach 58％at the substrate concentration of 30 g／L with an 1．7 fold increase compared with the aqueous system． Key wo