斑蝥素诱导人肺癌A549细胞凋亡及其分子机制的研究.pdfVIP

·330 · 2005 6 27 6 　Chin J Oncol, June 2005, Vol 27, No.6 ·· A549 张卫东　赵惠儒　阎影　王晓华　宗志红　刘莹 【】　　A549 。　MTT A549 ;、、、Annexin V- ITC DNA ;bcl-2、BaxSurvivin。 A549 ;A549 , ; G ;Annexin V- ITC 1 ;DNA ; Bax , bcl-2 Survivin 。　A549 , , Bax、bcl-2 Survivin 。 【】　;　A549 ;　 Apoptosis induced by cantharidin in human pulmonary carcinoma cells A549 and its molecular mechanisms ZHANG Wei-dong, ZHAO Hui-ru, YAN ying, WANGXiao-hua, ZONG Zhi-hong, LIU Ying.Department of Thoracic surgery, irst Affiliated Hospital, China Medical University, Shenyang 110001, China Corresonding author:ZHANG Wei-dong, E-mail:wdzhangdocter 62 @ - 【Abstract】　Objective　To investigate the apoptosis-inducing effect of cantharidin in human lung cancer cells A549 and its molecular mechanisms.Methods　MTT assay was used to determine A549 cells proliferation. Light and electron microscopy, ACScan, Annexin V- ITC staining and DNA gel electrophoresis were used to detect apoptosis.The expression of bcl-2, Bax and survivinwere examined by Western blot.Results　Cantharidin inhibited the proliferation of A549 cells.The cells treated with cantharidin showed a typical apoptotic morphology and hypodiploid peak before G phase.low cytometry analysis with annexin quantitatively further confirmed the 1 increase of cell apoptosis.DNA of treated A549 cells depicted a ladder pattern characteristic of apoptosis, indicating the presence of DNA fragmentation.Western blot assay showed that cantharidin increased the level of Bax expression and inhibited the level of bcl-2 and survivin expression.Conclusion　Cantharidin can induce A549 cells apoptosismainly via regulation of Bax, bcl-2 and survivin expression. 【Subject words】　Cantharidin;　Human pulmonary carcinoma A549 cell line;　Apoptosis